Isofraxidin inhibited proliferation and induced apoptosis via blockage of Akt pathway in human colorectal cancer cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Peng ShenRui Xie

Abstract

Isofraxidin (IF), a natural coumarin compound, has been reported to possess anti-cancer activity in human liver cancer. However, whether IF is involved in the regulation of colorectal cancer tumorigenesis and development has been not well elucidated. The cell proliferation were assessed by Cell Counting Kit-8 (CCK-8) and colony formation test, respectively. The transwell assays were conducted to estimate cell migration and invasion abilities. Further, cell apoptosis was evaluated by confocal microscopy analysis, flow cytometry detection and TdT-mediated dUTP Nick-End Labeling (TUNEL) method. Western blot were performed to detect the expression of related protein. Herein, the result indicated that IF remarkably bated cell proliferation in human colorectal cancer cells HT-29 and SW-480 in a dose- and time-dependent manner. In addition, IF treatment showed obvious inhibitory activity to cell colony formation in HT-29 and SW-480 cells. Confocal microscopy analysis and flow cytometry detection revealed that IF dramatically induced cell apoptosis in HT-29 and SW-480 cells compared with the control. And IF markedly decreased the expression of anti-apoptotic protein bcl-2, whereas the expression of pro-apoptotic proteins, including cas...Continue Reading

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Citations

Oct 10, 2018·Food & Function·Jialei JinHaixiao Liu
Jun 18, 2019·Clinical and Experimental Pharmacology & Physiology·Xinyue GuBinbin Cui
Mar 21, 2019·Journal of Cellular Biochemistry·Xiaoqiang SuXiangyang Wang
May 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohammad Bagher MajnooniMohammad Hosein Farzaei
Apr 22, 2020·International Immunopharmacology·Lei JinXiao-Ning Wu
Dec 22, 2020·Frontiers in Oncology·Yi WuGuojun Wu
Jul 18, 2020·Anti-cancer Agents in Medicinal Chemistry·Imran KhanIrfan A Ansari

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