Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR

Scientific Reports
Zahoor AhmadWubei Dong

Abstract

Plant growth-promoting bacteria (PGPB) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. In this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. Based on 16S rDNA and BIOLOG test analysis, the 330-2 strain was identified as Bacillus subtilis. The strain produced indole-3-acetic acid, siderophores, lytic enzymes and solubilized different sources of organic/inorganic phosphates and zinc. Furthermore, the strain strongly suppressed the in vitro growth of Rhizoctonia solani AG1-IA, Botrytis cinerea, Fusarium oxysporum, Alternaria alternata, Cochliobolus heterostrophus, and Nigrospora oryzae. The strain also significantly increased the seedling growth (ranging 14-37%) of rice and maize. Removing PCR analysis indicated that 114 genes were differentially expressed, among which 10%, 32% and 10% were involved in antibiotic production (e.g., srfAA, bae, fen, mln, and dfnI), metabolism (e.g., gltA, pabA, and ggt) and transportation of nutrients (e.g., fhu, glpT, and gltT), respectively. In summary, these results clearly indicate the effectiveness a...Continue Reading

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Citations

Oct 24, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Prity KushwahaRajesh Kumar Tiwari
Sep 25, 2019·World Journal of Microbiology & Biotechnology·Jianjun RenDongze Niu
Mar 22, 2020·Functional & Integrative Genomics·Nicolás D Franco-SierraJavier C Álvarez
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuanxiang PangYuanhong Xie

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Methods Mentioned

BETA
environmental stresses
PCR

Software Mentioned

BLAST
Mega5
Microlog SystemTm
STATISTIX
MEGA

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