PMID: 5325994Feb 1, 1966

Isolated fecal microorganisms capable of 7-alpha-dehydroxylating bile acids

The Journal of Experimental Medicine
B E GustafssonA Norman

Abstract

Strains of microorganisms capable of 7alpha-dehydroxylation of chenodeoxycholate were isolated from rat and human feces. All the strains were strictly anaerobic, non-motile, moderately themioresistant Gram-positive rods. They showed some saccharolytic properties with the production of both acid and gas. They were H(2)S-positive but indole-, skatole-, citrate-, catalase-, and oxidase-negative. The isolated strains capable of 7alpha-dehydroxylation of chenodeoxycholate were also able to oxidize the hydroxyl groups at C-3 and C-7 to keto groups. The following metabolites were isolated: 3-keto-7alpha-hydroxy-5beta-cholanoic acid, 3alpha-hydroxy-7-keto-5beta-cholanoic acid, 3alpha-hydroxy-5beta-cholanoic acid, and 3-keto-5beta-cholanoic acid. The isolated strains did not have the enzymes necessary for hydrolyzing conjugated bile acids. In mixed anaerobic cultures of fecal microorganisms, extensive reduction of the 3-keto group to the 3beta-hydroxyl group occurred. The microorganism(s) responsible for this reaction have as yet not been isolated.

References

Sep 1, 1954·Archives of Biochemistry and Biophysics·A H HALPERINP G SCHOLEFIELD
Mar 9, 1957·Nature·S HayakawaT EGUCHI
Mar 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·B E GustafssonA Norman
Apr 1, 1963·Archives of Biochemistry and Biophysics·J G HAMILTON
Jan 1, 1965·Pathologia et microbiologia·B BRZIN
Jun 1, 1955·Archives of Biochemistry and Biophysics·O HAYAISHIE F STOHLMAN
Oct 1, 1959·Archives of Biochemistry and Biophysics·E HEFTMANNE MOSETTIG
Jul 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·A Norman, M S SHORB
Apr 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·O W PORTMANB JORGENSEN

Citations

Sep 1, 1982·Digestive Diseases and Sciences·W H Bachrach, A F Hofmann
May 1, 1980·Physiology & Behavior·T M Lee, H Moltz
Apr 6, 2002·Environment International·Ian D BullRichard P Evershed
Aug 1, 1969·The British Journal of Nutrition·B E Gustafsson
Jul 1, 1989·The British Journal of Nutrition·C AndrieuxE Sacquet
Jan 1, 1995·Microbiology and Immunology·F Takamine, T Imamura
Apr 1, 1977·European Journal of Clinical Investigation·K HuibregtseG N Tytgat
Jul 1, 1993·Annals of the Rheumatic Diseases·A E HenrikssonA Uribe
Sep 1, 1968·The Journal of Clinical Investigation·P SamuelM Chafizadeh
Nov 1, 1971·The Journal of Clinical Investigation·J E King, L J Schoenfield
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Omry KorenFredrik Bäckhed
Jan 1, 1967·Acta Pathologica Et Microbiologica Scandinavica·T Midtvedt, A Norman
Jun 1, 1992·Scandinavian Journal of Gastroenterology·T OsnesM Osnes
Mar 1, 2011·Trends in Endocrinology and Metabolism : TEM·Thomas Greiner, Fredrik Bäckhed
Nov 1, 1971·The American Journal of Medicine·M P TyorL Lack
Dec 1, 1968·Journal of Pharmaceutical Sciences·R R Scheline
Apr 19, 2018·Gut Microbes·Aline Corado GomesJoão Felipe Mota
Jun 25, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lian Yee YipEric Chun Yong Chan
Sep 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M AngelicoA Blasi
Jan 1, 1972·Acta Pharmacologica Et Toxicologica·H A Soleim, R R Scheline
Jan 1, 1969·Acta Pathologica Et Microbiologica Scandinavica·A Trippestad
Jan 1, 1968·Acta Pathologica Et Microbiologica Scandinavica·T Midtvedt, A Norman
Jul 1, 1983·Scandinavian Journal of Gastroenterology·K O ViddalK Nygaard
Oct 1, 1986·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·B Carlstedt-DukeB E Gustafsson
Feb 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E HusebyeT Midtvedt
Jan 1, 1969·Acta Pathologica Et Microbiologica Scandinavica·T MidtvedtK Nygaard
May 8, 1999·The American Journal of Clinical Nutrition·R I MackieH R Gaskins
Jan 1, 1968·Acta Pathologica Et Microbiologica Scandinavica·T Midtvedt, A Norman
Jan 1, 1972·Acta Pathologica Et Microbiologica Scandinavica. Section B: Microbiology and Immunology·T Midtvedt, A Norman

Related Concepts

Bacteriological Techniques
Bile Salts
Carbon Isotopes
Chromatography
Feces
Mixed Function Oxygenases
Intestines

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