Isolated human islets require hyperoxia to maintain islet mass, metabolism, and function

Biochemical and Biophysical Research Communications
Hirotake KomatsuYoko Mullen

Abstract

Pancreatic islet transplantation has been recognized as an effective treatment for Type 1 diabetes; however, there is still plenty of room to improve transplantation efficiency. Because islets are metabolically active they require high oxygen to survive; thus hypoxia after transplant is one of the major causes of graft failure. Knowing the optimal oxygen tension for isolated islets would allow a transplant team to provide the best oxygen environment during pre- and post-transplant periods. To address this issue and begin to establish empirically determined guidelines for islet maintenance, we exposed in vitro cultured islets to different partial oxygen pressures (pO2) and assessed changes in islet volume, viability, metabolism, and function. Human islets were cultured for 7 days in different pO2 media corresponding to hypoxia (90 mmHg), normoxia (160 mmHg), and hyerpoxia (270 or 350 mmHg). Compared to normoxia and hypoxia, hyperoxia alleviated the loss of islet volume, maintaining higher islet viability and metabolism as measured by oxygen consumption and glucose-stimulated insulin secretion responses. We predict that maintaining pre- and post-transplanted islets in a hyperoxic environment will alleviate islet volume loss and m...Continue Reading

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Citations

Apr 30, 2017·Journal of Proteome Research·Marta Garcia-ContrerasPeter Buchwald
Sep 13, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H KomatsuY Mullen
Apr 6, 2018·Pancreas·Hirotake KomatsuYoko Mullen
May 22, 2018·Transplantation·Hirotake KomatsuYoko Mullen
Feb 14, 2019·Scientific Reports·Dina MyasnikovaJunji Fukuda
Dec 14, 2018·Biofabrication·Hirotake KomatsuYu-Chong Tai
Nov 4, 2020·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Hirotake KomatsuYoko Mullen
Aug 18, 2018·ACS Applied Materials & Interfaces·Manohar Prasad KoduriFan Gang Tseng
Oct 12, 2021·Cell Transplantation·Hirotake KomatsuIsmail H Al-Abdullah

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