Isolated kidney perfusion: the influence of pulsatile flow

Scandinavian Journal of Clinical and Laboratory Investigation
Charlotte von Horn, Thomas Minor

Abstract

Within the scope of transplantation research, ex vivo kidney perfusion has been proven an attractive model to study ischemia-reperfusion and preservation injury. Renal perfusion techniques also occupy scientists with the aim to optimize organ reconditioning and preparation prior to transplantation. This study investigated the influence of a pulsatile perfusion pattern that brings flow conditions closer to physiological situations, on renal perfusion characteristic and kidney function in the isolated perfused kidney. Kidneys were perfused via a roller pump at constant pressure set to 90 mmHg, or with addition of pulsatile pressure peaks (90/70 mmHg; 60/min) using an adjustable positive displacement pump. It was found that pulsatile pressure significantly enhanced renal flow rates as compared to non-pulsatile perfusion mode, especially after preceding preservation of the kidney by static cold storage. The improved vascular conductivity went along with a notable improvement of clearance of creatinine, sodium reabsorption and reduced tubular cell injury (Loss of fatty acid binding protein). The better vascular conductance upon pulsatile perfusion could be attributed to improved endothelial release of nitic oxide and reduced secreti...Continue Reading

References

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Apr 18, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Thomas MinorAnja Gallinat

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Citations

Dec 5, 2018·Artificial Organs·Thomas MinorAndreas Paul
Mar 23, 2019·Artificial Organs·Takuma MiyamotoKiyotaka Fukamachi
Jan 16, 2019·BioMed Research International·C SteichenR Thuillier
Oct 17, 2019·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Kazunori SanoTatsuya Shimizu
Feb 23, 2019·BioMed Research International·Maria Irene BelliniVassilios Papalois
Dec 12, 2018·Transplantation Direct·Isabel M A BrüggenwirthPaulo N Martins
Aug 2, 2019·Artificial Organs·Paria MahboubKorkut Uygun

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