Isolated limb perfusion with TNF alpha and melphalan in a rat osteosarcoma model: a new anti-tumour approach

European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
E R ManusamaA M Eggermont

Abstract

Isolated limb perfusion (ILP) with TNF alpha, IFN gamma and melphalan causes impressive tumour reduction in patients with irresectable soft tissue sarcomas with a high limb salvage rate. Since this therapy could be of value in patients with progressive osteosarcoma, we performed a study in an osteosarcoma tumour model in the rat. The ROS-1 osteosarcoma was implanted s.c. in the hind leg of WAG rats. Rats were divided in four groups: rats that underwent ILP with perfusate alone, TNF alpha alone, melphalan alone or their combination. Almost all rats, treated with a sham ILP or a perfusion with 40 micrograms melphalan, showed progressive disease (PD) (6/6 and 5/6). After perfusion with 50 micrograms TNF alpha alone a varied response was observed: 2/6 PD, 2/6 no change (NC) and 2/6 a complete remission (CR). After combined perfusion: 3/6 rats had a partial remission and 3/6 a CR. The best and most consistent responses are obtained by combining TNF alpha and melphalan. The discrepancy with the in vitro sensitivity of ROS-1 indicates that indirect effects are important in this tumour model.

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Jan 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D LienardF J Lejeune
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Jan 1, 1989·Cancer Immunology, Immunotherapy : CII·A L AsherS A Rosenberg
May 1, 1988·Annales De L'Institut Pasteur. Immunology·N BloksmaF M Hofhuis
Aug 1, 1988·The Journal of Experimental Medicine·P NawrothD Stern
Jun 19, 1986·The New England Journal of Medicine·M P LinkJ A Kirkpatrick
Jan 1, 1986·International Journal of Immunopharmacology·Y SohmuraH Furuichi
Nov 6, 1986·Journal of Immunological Methods·S M Kramer, M E Carver
Dec 1, 1982·Journal of Surgical Oncology·C BenckhuysenS C Van't Hoff
Jul 1, 1995·The Journal of Pathology·N RenardD J Ruiter
Mar 12, 1993·International Journal of Cancer. Journal International Du Cancer·S FolliJ P Mach

❮ Previous
Next ❯

Citations

Nov 4, 2004·Journal of the National Cancer Institute·Flavia BrunsteinTimo L M ten Hagen
Jul 10, 2003·The Lancet Oncology·Alexander M M EggermontTimo L M ten Hagen
Mar 28, 2008·Immunological Reviews·Timo L M ten HagenAlexander M M Eggermont
May 15, 2003·The Surgical Clinics of North America·Alexander M M EggermontTimo L M ten Hagen
Mar 8, 2006·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·D J GrünhagenA M M Eggermont
Feb 23, 1999·British Journal of Cancer·A H van der VeenA M Eggermont
Mar 13, 2019·The Journal of Clinical Investigation·Julia RühlChristian Münz
Apr 9, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·G TaegerS Ruchholtz
Apr 15, 2006·The Oncologist·Remco van HorssenAlexander M M Eggermont
Apr 9, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Timo L M Ten Hagen, Alexander M M Eggermont
Sep 2, 2018·Journal of Translational Medicine·Steven F JosephsAmir Jafri
Jan 9, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Narayanan KannanChandrasekaran Guruvayoorappan
May 2, 2019·The Journal of Clinical Investigation·Julia RühlChristian Münz
Feb 27, 2007·Archives of Biochemistry and Biophysics·Anje Cauwels, Peter Brouckaert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.