Isolated rat hepatocytes and 9000 g rat liver supernatant as metabolic systems for the study of the pharmacokinetics of barbiturates

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
T D Yih, J M van Rossum

Abstract

1. A method for metabolic studies with isolated rat hepatocytes has been developed. The rate of metabolism of a number of barbiturates has been studied both in hepatocytes and in 9000 g rat liver supernatant suspension. 2. The half-lives and clearance values, relative to heptabarbital, determined in hepatocytes were similar to the values obtained with isolated perfused rat liver. The values obtained with the 9000 g supernatant preparation were not. 3. In respect of metabolites formed, the metabolism of barbiturates in hepatocytes is comparable to that of the intact rat or liver perfusion. The metabolism in the 9000 g supernatant, however, is not.

References

Jun 1, 1976·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T D Yih, J M Rossum
Aug 1, 1974·Archives of Biochemistry and Biophysics·R N Zahlten, F W Stratman
Jul 10, 1974·Biochemical and Biophysical Research Communications·C von BahrS Orrenius
Jul 1, 1971·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T B VreeJ M van Rossum

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Citations

Mar 1, 1980·Archives of Toxicology·C LiddiardH Nau
Nov 1, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·S McLean
Nov 1, 1980·European Journal of Clinical Pharmacology·J Cobby, M C Makoid
Nov 15, 1981·Biochemical Pharmacology·D J BatesM Jarman
Jan 1, 1981·Toxicology·A P StammatiF Zucco
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J B Houston
Sep 1, 1981·Journal of Toxicology and Environmental Health·C A McQueen, G M Williams
Jan 1, 1982·Quarterly Reviews on Drug Metabolism and Drug Interactions·J R Fry
Jan 1, 1988·Drug Metabolism Reviews·M van der GraaffD D Breimer
Jan 1, 1980·Annals of the New York Academy of Sciences·R E McMahon
May 18, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Emilie Jigorel, J Brian Houston

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