Isolates of Theileria annulata collected from different parts of India show phenotypic and genetic diversity

Veterinary Parasitology
Anju ManujaAnil K Nichani

Abstract

Phenotypic and genetic polymorphism was studied amongst four Theileria annulata isolates collected from three different parts of India. Amongst various markers studied for the comparison of growth characteristics of schizont cell lines established from these isolates, viability, non-viability counts and nitric oxide (NO) production showed significant variation. A negative correlation was observed between NO production and mRNA expression for TNF-alpha, a potent proinflammatory cytokine related to the pathogenesis of the disease. Phenotypic polymorphism was also revealed by T. annulata schizont-specific monoclonal antibodies (Mabs), viz. 1C7, 1E11, 2G2 and EU-106, which recognized variable number of cells in indirect fluorescent antibody and indirect immunoperoxidase tests, when tested against the four T. annulata isolates collected from India. Genetic polymorphism was recognized amongst the four isolates by restriction digestion analysis of Tams-1 gene PCR products. These observations revealed that the four isolates of T. annulata are different from each other and might be expressing different antigenic determinants on their cell surface.

References

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Citations

Jul 30, 2014·International Immunopharmacology·Anju ManujaSuresh Chandra Yadav
Sep 1, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Neena GeorgeParesh Sharma
Mar 24, 2016·International Journal of Biological Macromolecules·Anju ManujaSuresh C Yadav
Nov 7, 2019·Scientific Reports·Thillaiampalam SivakumarNaoaki Yokoyama
Mar 6, 2021·Ticks and Tick-borne Diseases·Harshini Sheeja PrabhakaranManish Kumar
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Ahmed Magdy SelimAl Shymaa Al Sayed Shwareb

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