PMID: 8587858Oct 1, 1995Paper

Isolating fetal nucleated red blood cells from maternal blood: the Baylor experience--1995

Prenatal Diagnosis
J L SimpsonS Elias

Abstract

In our previous work we have isolated fetal cells from maternal blood and used fluorescent in situ hybridization (FISH) for chromosome-specific probes to detect aneuploidy. Current efforts in the Baylor College of Medicine programme are focusing on obtaining consistency in flow-sorting methodology and on determining sensitivity and specificity. To this end, systematic evaluation of five glycophorin A (gly A) antibodies all produced agglutination, leading us to abandon the use of gly A antibodies for positive selection of fetal cells. Conversely, we have found LDS-751 to be useful for nuclear selection. CD45 negative selection can best be accomplished by the use of flasks coated with goat antibodies against mouse antibodies. Positive selection by flow sorting for either CD71+ cells or gamma-globin-positive cells seems to be successful. Using these two approaches, we have recently detected male (fetal) cells in pregnancies in which the fetus was 46,XY in 10 of 18 and in 12 of 14 cases, respectively.

References

May 1, 1992·Prenatal Diagnosis·D H LedbetterK L Copeland
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Citations

Dec 13, 2000·Prenatal Diagnosis·A SekizawaD W Bianchi
May 8, 1998·Obstetrics and Gynecology·W HolzgreveS Hahn
Jul 31, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·S HahnW Holzgreve
Mar 22, 2001·Clinical Genetics·S S WachtelD Sammons
Jun 3, 1999·British Journal of Haematology·D W Bianchi
Dec 1, 1996·Clinical Obstetrics and Gynecology·C D SteeleL G Jackson
Feb 1, 1997·Obstetrical & Gynecological Survey·D M ShererM Y Divon
Apr 19, 2008·Obstetrical & Gynecological Survey·F M NdumbeJ C Konje
Apr 10, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Belén PrietoFrancisco V Alvarez
Jul 17, 2012·Best Practice & Research. Clinical Obstetrics & Gynaecology·Mahesh ChoolaniSinuhe Hahn
Dec 5, 1998·American Journal of Human Genetics·J C OosterwijkH J Tanke
Jun 22, 1999·American Journal of Medical Genetics·R Al-MuftiK H Nicolaides
Nov 25, 2003·Prenatal Diagnosis·Eva Irene Jauho, Mogens Havsteen Jakobsen
Jul 1, 1998·British Journal of Obstetrics and Gynaecology·W L MartinD T Liu
Apr 8, 2006·Current Opinion in Obstetrics & Gynecology·Farideh Z Bischoff, Joe Leigh Simpson
Dec 3, 2003·Hemoglobin·Yegneshwar S IyerDipika Mohanty

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