Isolating the metabolic pathways involved in the hepatoprotective effect of Muntingia calabura against CCl4-induced liver injury using LC/MS Q-TOF

Journal of Ethnopharmacology
M S RofieeM Z Salleh

Abstract

Muntingia calabura L. has been used in Southeast Asia and tropical America as antipyretic, antiseptic, analgesic, antispasmodic and liver tonic. This study aims to determine the acute toxicity and the metabolic pathways involved in the hepatoprotective mechanism of M. calabura. CCl4-induced hepatotoxic rat model was developed and a dose dependent effect of M. calabura was conducted. Body weight, food and water consumption were measured every day and rats were sacrificed to collect the serum samples at the end of the 10-days treatment. Liquid chromatography-mass spectrometry quadrapole time of flight (LC/MS-QTOF) combined with principal component analysis (PCA) were used to determine differentially expressed metabolites due to treatment with CCl4 and M. calabura extracts. Metabolomics Pathway Analysis (MetPA) was used for analysis and visualization of pathways involved. Body weight, food and water consumption were significantly decreased and histopathological study revealed steatosis in CCl4-induced rats. PCA score plots show distinct separation in the metabolite profiles of the normal group, CCl4-treated group and extract of M. calabura (MCME) pre-treated groups. Biomarkers network reconstruction using MetPA had identified 2 ma...Continue Reading

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Citations

Sep 23, 2018·Biomedical Chromatography : BMC·Thidarat IntararuchikulRossarin Tansawat
Mar 6, 2019·World Journal of Gastroenterology : WJG·Jiong YuLan-Juan Li
Jul 1, 2018·Natural Products and Bioprospecting·Qi ZhaoFei Li
Mar 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Xiao MengHua-Bin Li

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