PMID: 4292882Sep 1, 1967Paper

Isolation and amino acid composition of human angiotensin I

The Biochemical Journal
K ArakawaM Nakamura

Abstract

1. Angiotensin, the most powerful pressor substance known, suspected to be a causal substance in renal hypertension and previously isolated from animal sources, has now been isolated from human sources and the amino acid composition was analysed. 2. The procedures followed in the successful isolation of human angiotensin include: (a) preparation of stable materials to obtain maximum formation of human angiotensin; (b) a relatively selective adsorption of the formed angiotensin on Dowex 50W (X2); (c) gel filtration through Sephadex G-25; (d) cation-exchange chromatography on CM-cellulose; (e) anion-exchange gel filtration on DEAE-Sephadex A-25; (f) molecular-sieve chromatography through Bio-Gel P-2. 3. The homogeneity of the human angiotensin isolated was confirmed by paper and thin-layer chromatography and paper electrophoresis. 4. The biological activity observed indicates the substance isolated to be human angiotensin I. 5. The amino acid analysis suggested the following proportional composition: Asp, 1; Pro, 1; Val, 1; Ile, 1; Leu, 1; Tyr, 1; Phe, 1; His, 2; Arg, 1. This composition is similar to that of horse angiotensin I, i.e. isoleucine(5)-angiotensin I.

Citations

Nov 1, 1982·General and Comparative Endocrinology·G P GuthrieT A Kotchen
May 31, 1976·The American Journal of Medicine·L T SkeggsM Levine
Jan 1, 1971·American Heart Journal·H YamamotoM Nakamura
Nov 19, 2011·Journal of Anesthesia·Shigeru Saito
Nov 5, 2016·Journal of the American Society for Mass Spectrometry·Kevin Jeanne Dit FouqueCarlos Afonso
Aug 1, 1970·Circulation Research·G J MacdonaldW S Peart
Feb 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·T OnoK Arakawa
Nov 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·K ArawakaM Nakamura
Apr 30, 1981·Biochemical and Biophysical Research Communications·D A TewksburyJ Travis

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