PMID: 3319535Jan 1, 1987Paper

Isolation and amino acid composition of the isotypes of a rat Clara cell specific protein

Experimental Lung Research
G SinghS A Gottron

Abstract

A protein of molecular weight about 10,000 (Clara cell protein C) present in lung lavage fluid and specific to Clara cells has been shown to have three isotypes. The isotypes have been individually isolated and purified by a combination of molecular sieving, ion exchange chromatography, column chromatofocusing, and reverse phase chromatography. The protein was monitored by immunoblotting and its purity tested by silver staining of the gel following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. About 33.7% of the 10-kDa protein present in starting rat lavage was recovered in final pure preparation. The 10-kDa Clara cell protein amounted to 0.38% of the total protein in rat lung lavage fluid. The three isotypes were present in a ratio of 1.0:2.8:8.8, the amounts increasing with decreasing pI. None of the isotypes bound to concanavalin A. The amino acid compositions of the three isotypes were similar and were remarkable in that the contents of leucine, glutamic acid, aspartic acid, serine, and proline were high, tyrosine was present in low amounts, and methionine and histidine were absent.

References

Oct 1, 1975·Journal of Ultrastructure Research·C Kuhn, L A Callaway
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1979·The Journal of Clinical Investigation·G D MassaroL A Thet
Jun 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G SinghE J Riley
Jun 10, 1985·Biochimica Et Biophysica Acta·G SinghS A Gottron
Jan 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Singh, S L Katyal
Mar 28, 1980·Science·C J Serabjit-SinghC G Plopper

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Citations

Feb 12, 2009·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Henrik WidegrenLennart Greiff
Feb 2, 1999·American Journal of Respiratory and Critical Care Medicine·C Hermans, A Bernard
Nov 1, 1988·The American Review of Respiratory Disease·G SinghR J Mason
Oct 8, 1998·American Journal of Respiratory Cell and Molecular Biology·X L YaoJ H Shelhamer
Jan 1, 1991·Annals of the New York Academy of Sciences·G HelftenbeinM Beato
Feb 24, 2001·Annals of the New York Academy of Sciences·J NiJ Klug
Jul 6, 1990·Biochimica Et Biophysica Acta·G SinghM L Wong-Chong

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