PMID: 1346764Feb 15, 1992Paper

Isolation and amino acid sequence of a glutamic acid specific endopeptidase from Bacillus licheniformis

European Journal of Biochemistry
I Svendsen, K Breddam

Abstract

An endopeptidase cleaving specifically at the carboxyl side of acidic amino acid residues, preferentially at glutamic acid, has been isolated from a commercial extract obtained by fermentation with Bacillus licheniformis. Using ion-exchange chromatography and affinity chromatography on bacitracin-Sepharose, it was possible, from 100 ml commercial extract, to isolate 100 mg homogeneous enzyme in a yield of 50%. It is the first description of a large-scale isolation of a Glu/Asp-specific enzyme. The preparation was essentially free of contaminating activities. The isolated enzyme consists of one peptide chain of 222 amino acid residues and has a calculated molecular mass of 23,589 Da. The determined amino acid sequence shows similarity to the Glu/Asp-specific enzymes previously isolated from Staphylococcus aureus V8, Actinomyces sp. and Streptomyces thermovulgaris. The substrate preference of the enzyme has been investigated. Although non-specific cleavages were observed after prolonged hydrolysis at high enzyme concentrations the enzyme appears to be essentially specific for Glu-Xaa and Asp-Xaa, with strong preference for the former. The isolated enzyme exhibits a bell-shaped pH/activity profile with an optimum at pH 7.5-8.0. Th...Continue Reading

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Citations

Nov 1, 1996·Protein Science : a Publication of the Protein Society·H R StennickeK Breddam
Jan 1, 1994·Peptides·J Bongers, E P Heimer
Mar 12, 2013·Journal of Agricultural and Food Chemistry·Phanindra KalyankarRichard J FitzGerald
Sep 24, 2004·Journal of Molecular Microbiology and Biotechnology·Birgit VeithGerhard Gottschalk
Aug 17, 2010·Biological Chemistry·Toshio OnoTakayuki K Nemoto
Oct 20, 2000·Analytical Biochemistry·N Müller, F Bordusa
Jun 19, 2007·Protein Expression and Purification·Gudrun VogtentanzBrian F Schmidt
Jun 13, 2015·Critical Reviews in Food Science and Nutrition·Oscar L RamosAntónio A Vicente
Jul 23, 2005·Microbiological Research·Leila GabdrakhmanovaInna Leshchinskaya
Sep 22, 1992·Biochemistry·H Grøn, K Breddam
Jan 1, 2013·Genomics Insights·Archana Sharma, T Satyanarayana
Jan 8, 2020·Acta Crystallographica. Section D, Structural Biology·Kartik Manne, Sthanam V L Narayana
Sep 2, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·M HaenslerN Wehofsky
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·O Ibrahim-Granet, O Bertrand
May 1, 1996·Phytochemistry·A L Tan-WilsonK A Wilson
Feb 17, 2005·Journal of Agricultural and Food Chemistry·David SpellmanRichard J FitzGerald

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