Isolation and amino acid sequences of two Kunitz-type protease inhibitors from the sea anemone Anthopleura aff. xanthogrammica

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Sonomi MinagawaKazuo Shiomi

Abstract

Two protease inhibitors (AXPI-I and -II) were isolated from the sea anemone Anthopleura aff. xanthogrammica by a combination of acetone precipitation, gel filtration on Sephadex G-75, cation-exchange fast protein liquid chromatography (FPLC) on Mono S and reverse-phase HPLC on TSKgel ODS-120T. Both inhibitors are basic polypeptides, and their amino acid compositions are characterized by the presence of six half-Cys residues and the absence of Met and Trp. They are potently active against trypsin; inhibition of other serine proteases (alpha-chymotrypsin and elastase) is also displayed by only AXPI-I. However, the inhibitors show no affinity for metallo-proteases and cysteine proteases. Analyses of the N-terminal portion and enzymatic fragments established their complete amino acid sequences comprising 58 residues. The overall sequence homology and the conserved location of all half-Cys residues confirmed that the A. aff. xanthogrammica inhibitors belong to the Kunitz-type family.

References

Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·R S Norton
Jan 1, 1980·Toxicon : Official Journal of the International Society on Toxinology·D Mebs, E Gebauer
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·D MebsY Samejima
Jan 1, 1980·Annual Review of Biochemistry·M Laskowski, I Kato
Oct 20, 1995·The Journal of Biological Chemistry·H SchweitzM Lazdunski
May 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·O CastañedaE Karlsson

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Citations

Aug 8, 2002·Toxicon : Official Journal of the International Society on Toxinology·Margarita M MonastyrnayaEmma P Kozlovskaya
Apr 24, 2007·Biochemistry. Biokhimii︠a︡·I N SokotunE P Kozlovskaya
Jul 31, 2009·Annual Review of Genomics and Human Genetics·Bryan G FryRicardo C Rodríguez de la Vega
Sep 28, 2012·Marine Drugs·Bárbara FrazãoAgostinho Antunes
Jun 19, 2013·Marine Drugs·Caroline B F Mourão, Elisabeth F Schwartz
Jun 23, 2015·Toxins·Mahdokht JouiaeiBryan G Fry
Sep 26, 2015·Marine Drugs·Irina GladkikhEmma Kozlovskaya
Mar 21, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yamile GonzálezMaria A Chávez
Jun 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Joacir Stolarz OliveiraGlenn F King
May 29, 2015·PloS One·Karen Luna-RamírezLourival D Possani
Feb 5, 2008·Peptides·Tomohiro HonmaKazuo Shiomi
Dec 24, 2005·Marine Biotechnology·Tomohiro Honma, Kazuo Shiomi
Sep 9, 2011·Bioorganicheskaia khimiia·T I VakorinaE P Kozlovskaia
Jul 28, 2019·Marine Drugs·Santos Ramírez-CarretoClaudia Rodríguez-Almazán
Oct 11, 2020·Marine Drugs·Isabella D'Ambra, Chiara Lauritano
Jan 15, 2016·Bioorganicheskaia khimiia·A N Mikov, S A Kozlov

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