Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis

Molecular Microbiology
A SchulzW Schumann

Abstract

Bacillus subtilis contains at least three classes of heat-shock genes regulated by different mechanisms. We are studying class I heat-shock genes encoded by the operons dnaK and groE. These two operons are both expressed from a vegetative promoter, and their regulation involves a novel heat-shock element designated CIRCE. Here we show that induction of both operons results from enhanced synthesis of mRNA and is independent of de novo protein synthesis. To answer the question of whether dnaK is involved in the deregulation of the heat-shock response as reported for Escherichia coli, two different insertion mutations were isolated within the tetracistronic dnaK operon (orf39-grpE-dnaK-dnaJ). In one mutant a cat cassette was inserted at the beginning of orf39. Transcriptional analysis revealed that this mutation abolished expression of the whole operon. In contrast, the basal level of groE mRNA was significantly increased at 37 degrees C, followed by a prolonged delay in the shut off after temperature upshift. These data point to a crucial role for the orf39 gene in the regulation of class I heat-shock genes. In the other mutant an internal 0.8 kb Bg/II fragment of dnaK was replaced by the cat cassette. In contrast to E. coli dnaK...Continue Reading

References

Aug 4, 1978·Molecular & General Genetics : MGG·H Saito, H Uchida
May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Oct 1, 1992·Journal of Bacteriology·W ShiC A Gross
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S WicknerK McKenney
Dec 1, 1990·Journal of Bacteriology·J N EngelD Ganem
Mar 1, 1989·Journal of Bacteriology·C JohnsonC Georgopoulos
Dec 1, 1989·Current Opinion in Cell Biology·F M Watt
Dec 1, 1986·Journal of Bacteriology·D N ArnostiM J Chamberlin
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C Bardwell, E A Craig
Aug 1, 1993·Molecular Microbiology·B Bukau
Nov 1, 1994·Journal of Bacteriology·S RiethdorfM Hecker
Dec 1, 1993·Journal of General Microbiology·T EatonM Gasson
Mar 1, 1993·Journal of Bacteriology·M van AsseldonkG Simons
Feb 12, 2008·Current Protocols in Molecular Biology·S Watkins

❮ Previous
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Citations

Jan 21, 2003·Mass Spectrometry Reviews·Ran Rosen, Eliora Z Ron
Aug 9, 2011·Cell Stress & Chaperones·Toshifumi TomoyasuHideaki Nagamune
Nov 7, 1995·Biochimica Et Biophysica Acta·A J MacarioE Conway de Macario
Apr 15, 1996·FEMS Microbiology Letters·R Segal, E Z Ron
May 15, 2004·Research in Microbiology·Marinella S LaportMarcia Giambiagi-De Marval
Apr 27, 2002·Applied and Environmental Microbiology·Marcus KaltwasserWolfgang Schumann
Dec 19, 2006·Journal of Bacteriology·José A LemosRobert A Burne
Dec 30, 1999·Journal of Bacteriology·A C MinderF Narberhaus
Jul 14, 2000·Journal of Bacteriology·G HomuthW Schumann
Nov 12, 2002·Journal of Bacteriology·Adam C Wilson, Ming Tan
Jan 4, 2003·Journal of Bacteriology·Saskia VersteegWolfgang Schumann
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Franz Narberhaus
Feb 27, 2004·Cell Stress & Chaperones·Wolfgang Schumann
Dec 17, 2014·Mikrobiologiia·I E Vishniakov, S N Borkhsenius
Sep 29, 2009·Research in Microbiology·Noël Molière, Kürşad Turgay
May 17, 2003·BMC Bioinformatics·Michael M Mwangi, Eric D Siggia
Nov 26, 2013·Biochimica Et Biophysica Acta·Marie-Pierre Castanié-CornetPierre Genevaux
Aug 14, 2003·FEBS Letters·Hitoshi NakamotoKouji Kojima
Sep 22, 2018·Microbiology and Immunology·Toshifumi TomoyasuHideaki Nagamune
Jun 26, 1999·Molecular Microbiology·G HomuthW Schumann
Feb 13, 1999·Molecular Microbiology·F Narberhaus
Apr 13, 2017·FEMS Microbiology Reviews·Davide Roncarati, Vincenzo Scarlato
Sep 5, 2007·Microbiology·Vineet K SinghNeal R Chamberlain
Sep 8, 2017·Frontiers in Microbiology·Amy L BottomleyElizabeth J Harry
Jun 6, 1998·Journal of Bacteriology·A MogkU Völker
Jul 31, 1998·Journal of Bacteriology·B KochK Hammer

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