Isolation and Analysis of Plasma-Derived Exosomes in Patients With Glioma

Frontiers in Oncology
Luz M Cumba GarciaIan F Parney

Abstract

Gliomas including glioblastoma (GBM) are the most common primary malignant brain tumors. Glioma extracellular vesicles (EVs) including exosomes have biological effects (e.g., immunosuppression) and contain tumor-specific cargo that could facilitate liquid biopsies. We aimed to develop a simple, reproducible technique to isolate plasma exosomes in glioma patients. Glioma patients' and normal donors' plasma exosomes underwent brief centrifugation to remove cells/debris followed by serial density gradient ultracentrifugation (DGU). EV size/concentration was determined by nanoparticle tracking. Protein cargo was screened by array, western blot, and ELISA. Nanoscale flow cytometry analysis quantified exosome and microvesicle populations pre- and post-DGU. One-step DGU efficiently isolates exosomes for nanoparticle tracking. Wild type isocitrate dehydrogenase glioma patients' (i.e., more aggressive tumors) plasma exosomes are smaller but higher concentration than normal donors. A second DGU efficiently concentrates exosomes for subsequent cargo analysis but results in vesicle aggregation that skews nanoparticle tracking. Cytokines and co-stimulatory molecules are readily detected but appeared globally reduced in GBM patients' exosome...Continue Reading

References

Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Dec 14, 2006·Histology and Histopathology·M NakamuraN Konishi
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Jun 19, 2009·Cell Cycle·Khalid Al-NedawiJanusz Rak
Mar 1, 2012·Seminars in Cancer Biology·Paola FilipazziVeronica Huber
Aug 31, 2012·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Nicolas DeaDavid Fortin
Apr 3, 2013·Expert Review of Neurotherapeutics·Tim J KruserH Ian Robins
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Oct 18, 2013·The New England Journal of Medicine·Sunit Das, Philip A Marsden
Mar 19, 2014·Pharmacogenomics and Personalized Medicine·Jasmina S RedzicMichael W Graner
Oct 16, 2014·Journal of Extracellular Vesicles·Jan Van DeunAn Hendrix
Mar 1, 2015·Seminars in Cell & Developmental Biology·David W GreeningWeisan Chen
Apr 24, 2015·Journal of Neuro-oncology·Johnny C AkersClark C Chen
May 12, 2015·Nature Communications·Huilin ShaoRalph Weissleder
Jun 11, 2015·The New England Journal of Medicine·Jeanette E Eckel-PassowRobert B Jenkins
Jun 30, 2015·Molecular Aspects of Medicine·Keon MahmoudiCostas Hadjipanayis
Jul 22, 2015·Journal of Extracellular Vesicles·Richard J LobbAndreas Möller
Sep 20, 2015·Neuro-oncology·Justin E HellwinkelMichael W Graner
Nov 12, 2016·Cancer Gene Therapy·L SaadatpourH Mirzaei
Mar 4, 2017·Theranostics·Pin LiZhiqiang Gao
Oct 28, 2017·Nature Communications·Zhiyun WeiAnna M Krichevsky
Mar 14, 2018·Science Advances·Franz L RicklefsE Antonio Chiocca
Dec 19, 2018·Neuro-oncology·Kyle FraserRalph Weissleder

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Citations

Apr 21, 2020·Current Pharmaceutical Design·Guifang ZhaoWenliang Li
Jan 9, 2020·Journal of Neuro-oncology·Tristan de MooijIan F Parney
Aug 23, 2020·Scientific Reports·Hae Hyun JungYoung-Hyuck Im
Jan 7, 2021·Neuropathology and Applied Neurobiology·Ana Belén Díaz MéndezMaria G Rizzo
Dec 4, 2019·Methods : a Companion to Methods in Enzymology·Jin GaoZhenjia Wang
Nov 18, 2020·Diagnostics·Marta ZaràSilvia S Barbieri
Apr 4, 2021·International Journal of Molecular Sciences·Sara Monteiro-ReisCarmen Jerónimo
Apr 13, 2021·Biosensors & Bioelectronics·Mehdi MohammadiAmir Sanati-Nezhad
Jun 22, 2021·Frontiers in Oncology·Hamza AliMyron G Best
Aug 9, 2021·Drug Delivery and Translational Research·Gayatri Gopal ShetgaonkarRupesh Kalidas Shirodkar
Sep 29, 2021·Neuro-oncology·Massimiliano Del BeneGiuliana Pelicci

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Methods Mentioned

BETA
surgical resection
blood draw
biopsies
PCR
size exclusion chromatography
density gradient ultracentrifugation
flow cytometry
ELISAs
ELISA
protein array

Software Mentioned

GraphPad Prism

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