Isolation and analysis of ribonucleic acids from skeletal tissues.

Analytical Biochemistry
G G NemethM E Bolander

Abstract

We report a method for the isolation of total cellular RNA from mineralized or cartilaginous tissues. The procedure accommodates the large amount of hydroxyapatite and high buoyant density proteoglycans present in skeletal tissue samples, as well as the low cell density characteristic of these tissues. The procedure can be reliably used for processing a large number of small (100-800 mg) tissue samples. Tissues are homogenized in guanidine hydrochloride solution, then centrifuged at low speed, and filtered to remove the nonsolubilized extracellular matrix proteins. Subsequent high speed density gradient centrifugation produces a high yield of RNA (0.2-0.6 micrograms RNA/mg tissue) which is precipitated in a low pH sodium acetate solution. RNA extracted by this method has been analyzed for the expression of various genes by Northern blotting. In addition to mRNAs of bone- and cartilage-specific proteins, messenger RNA for growth factors, proto-oncogenes, and heat shock proteins can be detected.

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A ThiedeG A Rodan
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K DoegeY Yamada
Jun 4, 1974·Biochemistry·V GlisinC Byus
May 1, 1984·Analytical Biochemistry·J Meinkoth, G Wahl
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1984·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·F Bonnarens, T A Einhorn

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Citations

Sep 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S JingushiM E Bolander
Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C SchmidE R Froesch
Jan 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T Laitala, K Väänänen
Oct 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F J BuchinskyS Epstein
Apr 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N EndoA Schmidt
Oct 6, 2001·Biochemical and Biophysical Research Communications·H TanakaC T Liang
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K UrabeY Iwamoto
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B KinnerM Spector
Oct 14, 2003·Bone·Lorenz MeinelBrigitte von Rechenberg
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SchmidtG A Rodan
Mar 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T ShutoM E Bolander
Sep 1, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R t BallockL M Mink
Aug 21, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Vera LuginbuehlHans P Merkle
Aug 5, 2003·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Ken UrabeMark E Bolander
Dec 1, 1990·Molecular and Cellular Probes·S P ScullyM E Bolander
Jul 21, 2011·Biochemical and Biophysical Research Communications·H TanakaC T Liang
Apr 1, 1992·Analytical Biochemistry·M E AdamsL J Sandell
Sep 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K H YangM E Bolander
Sep 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S JingushiM E Bolander
Jan 13, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K UrabeM E Bolander
Apr 1, 1997·The Journal of Clinical Endocrinology and Metabolism·D A SassS Epstein
Feb 14, 1994·FEBS Letters·A F OberhauserJ M Fernandez
Aug 16, 2000·Clinical Orthopaedics and Related Research·M UchinoM Itoman
Jan 1, 1990·Annals of the New York Academy of Sciences·M E JoyceM E Bolander
Oct 3, 2002·Clinical Orthopaedics and Related Research·Charles H RundleDavid J Baylink
Feb 11, 1998·Experimental Cell Research·B JohnstoneJ U Yoo
Dec 15, 1992·Biochemical and Biophysical Research Communications·T IkedaC Shigeno

❮ Previous
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