Isolation and analysis of the vaccine potential of an attenuated Edwardsiella tarda strain

Vaccine
Yun SunLi Sun

Abstract

Edwardsiella tarda is an important aquaculture pathogen that can infect a wide range of marine and freshwater fish worldwide. In this study, a modified E. tarda strain, TX5RM, was selected by multiple passages of the pathogenic E. tarda strain TX5 on growth medium containing the antibiotic rifampicin. Compared to the wild type strain, the rifampicin-resistant mutant TX5RM (i) shows drastically increased median lethal dose and reduced capacity to disseminate in and colonize fish tissues and blood; (ii) exhibits slower growth rates when cultured in rich medium or under conditions of iron depletion; and (iii) differs in the production profile of whole-cell proteins. The immunoprotective potential of TX5RM was examined in a Japanese flounder (Paralichthys olivaceus) model as a vaccine delivered via intraperitoneal injection, oral feeding, bath immersion, and oral feeding plus immersion. All the vaccination trials, except those of injection, were performed with a booster at 3-week after the first vaccination. The results showed that TX5RM administered via all four approaches produced significant protection, with the highest protection levels observed with TX5RM administered via oral feeding plus immersion, which were, in terms of re...Continue Reading

References

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Mar 6, 2007·Applied and Environmental Microbiology·Wei-wei Zhang, Li Sun
Nov 13, 2008·Diseases of Aquatic Organisms·Dong Jin LeeKi Hong Kim
Sep 29, 2009·Veterinary Microbiology·Kun SunLi Sun
Oct 29, 2009·The Journal of Antimicrobial Chemotherapy·Sylvia ValdezateJuan A Saéz-Nieto

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Citations

Dec 3, 2011·Developmental and Comparative Immunology·Irene SalinasJ Oriol Sunyer
Nov 12, 2015·Frontiers in Immunology·Hetron Mweemba Munang'anduØystein Evensen

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