Isolation and anti-inflammatory activity of colchicinoids from Gloriosa superba seeds

Pharmaceutical Biology
C S JoshiC S Mathela

Abstract

Gloriosa superba L. (Liliaceae) seeds, known as "kalihari" (Hindi), were phytochemically investigated for colchicine (well known for gout treatment) and other related alkaloid content. Colchicine, 2- demethylcolchicine, 3-demethylcolchicine, and N-formyl-N-deacetylcolchicine were alkaloids isolated from the seeds. The isolated samples have been standardized for their purity with respect to the reference standard using HPLC. The structures were confirmed by NMR spectroscopy and were analyzed by spiking them along with colchicine reference by HPLC. The purity of colchicine, 2- demethylcolchicine, 3- demethylcolchicine and N-formyl-N-deacetylcolchicine were 99.82, 96.78, 98.71, and 98.13% respectively. The compounds were subjected to an anti-inflammatory study by using the formaldehyde inflammagen-induced inflammation model. Oral administration of colchicine at 2, 4, and 6 mg/kg body weight resulted in 48.9, 68.7, and 79.1% inhibition respectively, while 30.9% inhibition was seen in the phenylbutazone 100 mg/kg treated group once daily for a period of 4 days. The results clearly indicated that the colchicine is more effective as an anti-inflammatory agent compared with phenylbutazone, the standard drug used in the study, whereas t...Continue Reading

References

Jun 30, 1975·Annals of the New York Academy of Sciences·W J MalaisseL Orci
Jun 30, 1988·The New England Journal of Medicine·D KershenobichM Rojkind
May 1, 1981·Agents and Actions·P Jain, N K Khanna
Dec 1, 1981·Annals of the Rheumatic Diseases·J N FordhamH L Currey
Aug 1, 1995·The Journal of Clinical Investigation·B N CronsteinG Weissmann
Nov 1, 1993·Annals of the Rheumatic Diseases·R J McKendryA al-Awadhi
Feb 1, 1993·Arthritis and Rheumatism·B N Cronstein, G Weissmann
Sep 3, 1998·Seminars in Arthritis and Rheumatism·E Ben-Chetrit, M Levy
Aug 28, 1999·Journal of Ethnopharmacology·H SüleymanA Gepdiremen
May 10, 2007·Natural Product Research·S KannanK Kumaragurubaran

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Citations

Jan 17, 2012·Journal of Ethnopharmacology·Dinesh KumarOm Prakash
Oct 1, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ashwell R NdhlalaJohannes Van Staden
Oct 5, 2011·Asian Pacific Journal of Tropical Medicine·Chidambaram KumarapppanKarpagam Kumarasunderi
Aug 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Acharya BalkrishnaC S Joshi
Nov 10, 2010·Fitoterapia·Sonali Jana, G S Shekhawat

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