PMID: 6971652Jan 21, 1981Paper

Isolation and biochemical characterization of leukemia-associated inhibitory activity that suppresses colony and cluster formation of cells

Biochimica Et Biophysica Acta
J BognackiJ Lobue

Abstract

Leukemia-associated inhibitory activity suppresses colony and cluster formation in vitro cells derived from granulocyte-macrophage progenitor cells of normal donors. It does not inhibit these same progenitor cells from patients with leukemia and it may contribute to the proliferative advantage leukemia cells appear to possess over normal hematopoietic cells during acute leukemia. The inhibitory activity was isolated by a combination of procedures including: ultracentrifugation, Sephadex G-200, carboxymethylcellulose, SDS-polyacrylamide gel electrophoresis, thin-layer and preparative isoelectric focusing and concanavalin A-Sepharose. Leukemia-associated inhibitory activity was characterized as a glycoprotein. it was inactivated by trypsin, chymotrypsin, pronase and periodate treatment. It bound to and was eluted by alpha-methylmannose from concanavalin A-Sepharose columns and had an apparent Mr range of 450-550 000 and an isoelectric focus value between pH 4.6 and 4.9. Crude leukemia associated inhibitory activity was temperature sensitive but the more purified preparations were heat stable.

References

Oct 1, 1978·The Journal of Experimental Medicine·H E BroxmeyerM de Sousa
Aug 16, 1979·The New England Journal of Medicine·H E BroxmeyerM A Moore
Feb 1, 1978·The American Journal of Medicine·M J Cline, D W Golde
Jan 1, 1979·Advances in Immunology·D C Morrison, J L Ryan
Mar 1, 1978·Journal of the National Cancer Institute·H E BroxmeyerA S Moore
Mar 1, 1978·Journal of the National Cancer Institute·H E BroxmeyerM A Moore
Apr 24, 1974·Journal of Chromatography·J Turková
Sep 15, 1973·Journal of Molecular Biology·F W Studier

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