PMID: 6112016Mar 26, 1981Paper

Isolation and characterisation of a non-polyadenylated mRNA species with an affinity for poly(adenylic acid) from Friend leukaemia cells

Biochimica Et Biophysica Acta
P K Katinakis, R H Burdon

Abstract

Utilizing the technique of poly(A)-Sepharose affinity chromatography, it is possible to isolate a novel class of RNA molecules from polysomes of Friend leukaemia cells. These RNA species display messenger RNA-like behaviour. They are released from polysomes on treatment with EDTA and are able to direct polypeptide synthesis in a cell-free protein synthesising system. They appear to be distinct from the polyadenylated mRNAs, as judged by their lack of a 3'-terminal poly(A) tract, by their different size distribution, by their unusual base composition, by the presence of a possible 'uridylate rich' region towards their 3'-end, by their low sequence homology to polyadenylated mRNAs and by the difference in at least some of their translation products.

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Citations

Dec 10, 2013·BioMed Research International·Elaine Regina Delicato de AlmeidaMaria Angelica Ehara Watanabe
Jan 15, 1984·Journal of Molecular Biology·D Tautz, M Renz

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