Isolation and characterisation of equine dendritic cells

Veterinary Immunology and Immunopathology
E M SiedekA Hamblin

Abstract

Despite their important role in initiating T-cell responses in other species, dendritic cells have not been studied in the horse. A method for isolating blood dendritic cells by adherence and metrizamide gradients was adapted to equine cells. A number of monoclonal antibodies (mAbs), including some which label dendritic cells in other species, were tested for immunochemical reactivity with the isolated blood dendritic cells, and sections of lymph node and spleen. 62 +/- 6% of the isolated blood cells were MHC Class II positive and had typical dendritic cell morphology and only 4 +/- 2% contained non-specific esterase, a marker of mature macrophages. These dendritic cells also expressed MHC Class I, LFA-1, EqWC1 and EqWC2. Amongst the potentially cross-reactive antibodies a mAb against bovine CD1b was the most interesting by staining lymph node, but not blood, dendritic cells. Monoclonal antibodies against equine CD5 (T-cells), surface immunoglobulin (B-cells) and macrophages (CZ2.2) were used to enumerate the contaminating cells in preparations from blood by flow cytometry. 39 +/- 7% of the cells did not express T and B cell markers or CZ2.2 but were large and MHC Class II positive. Comparison of immuno-chemistry and flow data,...Continue Reading

References

Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·R TsunodaM Kojima
Dec 1, 1992·European Journal of Immunology·D J McKeeverW I Morrison
May 1, 1992·The Journal of Experimental Medicine·K InabaG Schuler
Jan 1, 1991·Veterinary Immunology and Immunopathology·C J HowardJ J Letesson
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P S Freudenthal, R M Steinman
Oct 1, 1989·The Journal of Experimental Medicine·R BujdosoI McConnell
Aug 1, 1986·Journal of Immunogenetics·T CrepaldiD F Antczak
Jul 1, 1987·Digestive Diseases and Sciences·J A Walker-Smith, T T MacDonald
Apr 1, 1982·The Journal of Experimental Medicine·W C Van VoorhisG Kaplan
Jul 1, 1994·Veterinary Immunology and Immunopathology·J KyddM A Holmes
Sep 1, 1994·American Journal of Respiratory Cell and Molecular Biology·J M van HaarstH A Drexhage
Nov 1, 1993·American Journal of Respiratory Cell and Molecular Biology·C E HavenithE C Hoefsmit
Mar 1, 1993·American Journal of Respiratory Cell and Molecular Biology·C E HavenithE C Hoefsmit
Apr 1, 1996·The Journal of Experimental Medicine·J M Austyn
Jun 1, 1996·Immunology Today·J H PetersF Steinbach

❮ Previous
Next ❯

Citations

Mar 11, 2004·Veterinary Immunology and Immunopathology·K J RickardsF M Cunningham
Jun 20, 2002·Veterinary Immunology and Immunopathology·Anna VecchioneAnne Hamblin
Feb 15, 2003·Veterinary Immunology and Immunopathology·E JohanssonC Fossum
Jan 27, 2007·Journal of Immune Based Therapies and Vaccines·M Julia B F FlaminioMary Beth Matychak
Apr 1, 2008·Journal of Immunological Methods·Emilio Del CachoHyun S Lillehoj
Mar 3, 2015·Veterinary Immunology and Immunopathology·Gerardo Pavel Espino-SolisLourival Domingos Possani
Aug 15, 2000·Placenta·A C Enders, I K Liu
Jul 16, 2005·Pathologie-biologie·C Miranda de CarvalhoL Chabanne
Dec 6, 2001·Veterinary Pathology·S M Rhind
Nov 8, 2017·BMC Veterinary Research·Yao LeeGisela Soboll Hussey
Jan 5, 2002·Equine Veterinary Journal·S C BellS D Carter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.