Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study

Stem Cells International
Norhayati YusopRachel J Waddington

Abstract

Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs). This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation. CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs. Both populations exhibited consistent telomere lengths (12-17 kb), minimal senescence, and positive telomerase expression. CB-MSCs (PD15) had significantly lower CFEs than PD50. CB-MSCs and BM-MSCs both expressed MSC (CD73/CD90/CD105); embryonic (Nanog) and osteogenic markers (Runx2, osteocalcin) but no hematopoietic markers (CD45). CB-MSCs (PD15) strongly expressed Oct4 and p16INK4A. At early PDs, CB-MSCs possessed a strong osteogenic potency and low potency for adipogenesis, whilst BM-MSCs possessed greater overall bipotentiality for osteogenesis and adipogenesis. At PD50, CB-MSCs demonstrated reduced potency for both osteogenesis and adipogenesis, compared to BM-MSCs at equivale...Continue Reading

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Citations

Nov 14, 2019·Cytotechnology·Nadia S MahmoudMohamed A Tantawy
Jan 19, 2020·Tissue Engineering. Part B, Reviews·Steven James AveryRachel Jane Waddington
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Baraa NoueihedSylvain Chemtob
Aug 7, 2021·International Journal of Biological Macromolecules·Özge Karabıyık AcarGamze Torun Kose
Sep 28, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Lubomir MedveckyVladimir Girman

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Methods Mentioned

BETA
Chip
chips
light microscopy
Assay

Software Mentioned

SPSS
Quantity One Image Analysis

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