Isolation and characterisation of poliovirus mutants resistant to heating at 50 degrees Celsius for 30 min

Journal of Medical Virology
Hiroshi ShiomiKoki Taniguchi

Abstract

Poliovirus is heat-labile; on heating at 50 degrees Celsius for 30 min its infectivity decreases drastically and its antigenicity reverts from N to H. However, mutants resistant to heating at 50 degrees Celsius for 30 min from the Sabin 1 and 2 viruses were isolated by repeating the process of incubation of the virus stock at 50 degrees Celsius for 30 min and multiplication of the remaining virus in a cell culture. The isolated mutants were stable genetically, and maintained the rct and d markers of the parent virus. On electron microscopical examination, the mutants were observed to retain the intact morphology after being heated at 50 degrees Celsius for 30 min, while the parent virus was converted to empty particles devoid of RNA under the same conditions. On determination of the nucleotide sequence of the P1 region, a single nucleotide sequence substitution was detected at nucleotide no. 2741, resulting in an amino acid change from valine to alanine at the 87th position of VP1. This amino acid might be associated with the heat-resistance of the mutants. Furthermore, it was found that the thermostable mutants obtained in this study, which are resistant to "high" temperature (50 degrees Celsius) for a short time (30 min), wer...Continue Reading

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Citations

Nov 29, 2008·Archives of Virology·Andreas Sauerbrei, P Wutzler
Jul 4, 2013·Veterinary Microbiology·Jacquelyn HorsingtonCarol A Hartley
Dec 15, 2010·International Journal of Molecular Sciences·Jiu-Hong LiangJi-Hong Meng
Dec 9, 2016·Journal of Virology·Oluwapelumi O AdeyemiDavid J Rowlands
Dec 14, 2018·Journal of Virology·Oluwapelumi O AdeyemiNicola J Stonehouse
Feb 23, 2019·Journal of Virology·Anthony J Snyder, Pranav Danthi
Jan 9, 2020·Food and Environmental Virology·P WaldmanS Wurtzer
Feb 28, 2007·The Journal of General Virology·Roberto MateoMauricio G Mateu
Mar 27, 2021·Scientific Reports·Florian MattenbergerRon Geller
Sep 3, 2020·Environmental Science & Technology·Anna CarratalàTamar Kohn
Feb 22, 2018·Environmental Science & Technology·Simon MeisterTamar Kohn

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