Isolation and characterisation of six putative wheat cell wall-associated kinases

Functional Plant Biology : FPB
Yong LiuAimin Zhang

Abstract

The plant cell wall-associated kinase (WAK) and WAK-like kinase (WAKL) make up a unique group in the receptor-like protein kinase (RLK) superfamily. Previous studies on Arabidopsis have revealed that the WAK gene family members play an important role in both cell elongation and stress response signalling. Here we show that four putative WAKs (TaWAK1, TaWAKL2, TaWAKL3, and TaWAK4) and two WAKLs (TaWAKL1 and TaWAKL2) were isolated from wheat based on the DNA sequence similarity and the protein structure conservation of Arabidopsis WAKs genes. TaWAK1, TaWAK2, TaWAK3 and TaWAKL1 each encode a putative intact protein with the characteristic of the WAK / WAKL gene family members, except for the abbreviated TaWAK4 and TaWAKL2 which were caused by nucleotide mutation and alternative splicing, respectively. Southern analysis revealed that TaWAKL1, TaWAK1, TaWAK2 and TaWAK3 are all multiple-copy members. Real-time PCR analysis revealed that the TaWAK1 and TaWAK3 displayed similar expression patterns, while expressions of TaWAKL1, TaWAKL2, and TaWAK2 were organ specific. Further, we analysed the conservation of introns and intron-exon structure and the putative protein structures between wheat and Arabidopsis, which showed the putative wh...Continue Reading

References

Apr 1, 1987·The Journal of Cell Biology·F BlasiK Danø
Apr 14, 1995·Science·S Artavanis-TsakonasM E Fortini
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M LongW Gilbert
Aug 16, 1996·The Journal of Biological Chemistry·Z H HeB D Kohorn
Mar 11, 1998·Journal of Molecular Evolution·A FedorovE Grigor'ev
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·Z H HeB D Kohorn
Apr 30, 1999·Science·S Artavanis-TsakonasR J Lake
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·P M SchenkJ M Manners
May 4, 2001·The Journal of Biological Chemistry·A R ParkO K Park
Aug 18, 2001·Current Biology : CB·D J Cosgrove
Sep 7, 2001·Current Opinion in Cell Biology·B D Kohorn
Jun 18, 2002·Plant Physiology·Joseph A Verica, Zheng-Hui He
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Philip W Becraft
Mar 21, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H-Q LingB Keller
Jul 23, 2003·Current Opinion in Plant Biology·Erin R Morris, John C Walker
Dec 19, 2003·Nucleic Acids Research·Ivica LetunicPeer Bork
Mar 17, 2005·Plant & Cell Physiology·Annabelle Decreux, Johan Messiaen
Jul 1, 2004·Functional Plant Biology : FPB·Marc H EllisRichard A Richards
Nov 1, 2005·Functional Plant Biology : FPB·Dorthe VilladsenTom Hamborg Nielsen

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Datasets Mentioned

BETA
DQ013355
DQ013359

Methods Mentioned

BETA
transgenic
PCR
electrophoresis

Software Mentioned

Mega
ClustalW
DNAMAN
SignalP

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