Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins

Gene
A R LongD Shepherd

Abstract

Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26-29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1991·Seminars in Cell Biology·A B Futcher
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 11, 1989·Nucleic Acids Research·A E Gorbalenya, E V Koonin
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jul 11, 1984·Nucleic Acids Research·P O O'Connell, M Rosbash
Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Confalonieri, M Duguet
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·J ShiM K Bhattacharyya
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B OhanaC A Rosen

❮ Previous
Next ❯

Citations

Dec 31, 2002·Current Opinion in Structural Biology·Andrei N Lupas, Jörg Martin
Jun 20, 2006·Biochemical and Biophysical Research Communications·Masami NagahamaMitsuo Tagaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.