PMID: 9426628Jan 14, 1998Paper

Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones encoding proteins involved in magnesium chelation into protoporphyrin IX

Plant Molecular Biology
E KruseB Grimm

Abstract

We have identified cDNA clones encoding the two Mg chelatase subunits CHL I and CHL H from tobacco (Nicotiana tabacum) by screening a cDNA library with homologous cDNA fragments from Arabidopsis thaliana. A full-length Chl I cDNA clone encodes a peptide with 426 amino acids. The entire cDNA sequence encoding 1382 amino acid long CHL H was obtained by extension of a truncated cDNA fragment using the 'rapid amplification of cDNA ends' (RACE) method. Both genes Chl I and Chl H were strongly expressed in young leaves and to a lesser extent in mature leaves. Only traces of both transcripts were found in flowering organs. Southern blot analysis suggests that CHL I is encoded by a single gene and CHL H most likely by several genes.

Citations

Jun 16, 2007·Proteomics·Pingfang YangShihua Shen
May 30, 2003·Plant & Cell Physiology·Ki-Hong JungGynheung An
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S GräfeF Hänel
Sep 28, 2004·The Plant Journal : for Cell and Molecular Biology·Choong-Min RyuKirankumar S Mysore
Aug 12, 2021·Scientific Reports·Yoshihiko NanasatoToru Taniguchi

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