PMID: 8588213Jul 1, 1995Paper

Isolation and characterisation of two haemorrhagic proteins (HTa and HTb) from the venom of Bitis gabonica (Gaboon viper)

Toxicon : Official Journal of the International Society on Toxinology
N A MarshE A Bennett

Abstract

Two distinct haemorrhagic proteinases, HTa and HTb, were isolated from the venom of Bitis gabonica by gel filtration and ion-exchange chromatography with native mol. wts of 180,000 and 111,000, respectively. After reduction with dithiothreitol, smaller mol. wts of 77,600 and 69,200 were recorded for HTa and HTb, suggesting that under native conditions the haemorrhagins exist as dimeric molecules. Both toxins possessed caseinolytic and collagenase activity although HTa was 15-36 times more potent than HTb with respect to collagenase activity. No zinc could be detected in the toxins; however, dialysis against ethylenediamine tetracetic acid (EDTA) reduced caseinolytic activity, suggesting the dependence of the latter on other metal ions. HTa and HTb had a marked effect on the intrinsic cascade coagulation mechanism (factors IX, XI and XII) but no effect on the final common coagulation pathway (factor X and prothrombin). Light and electron microscopical studies demonstrated that both HTa and HTb caused organ-specific lesions, with the lungs, diaphragm and body wall muscle being most affected. HTa caused widespread haemorrhage whilst HTb caused discrete focal lesions near the site of injection and elsewhere. However, both toxins ap...Continue Reading

References

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Citations

Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Adrijana LeonardiIgor Krizaj
Apr 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·N A MarshE A Bennett
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Neville Marsh, Vaughan Williams
Mar 16, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Neville MarshMichael Touger
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·José María GutiérrezCecilia Díaz

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