Abstract
Two distinct haemorrhagic proteinases, HTa and HTb, were isolated from the venom of Bitis gabonica by gel filtration and ion-exchange chromatography with native mol. wts of 180,000 and 111,000, respectively. After reduction with dithiothreitol, smaller mol. wts of 77,600 and 69,200 were recorded for HTa and HTb, suggesting that under native conditions the haemorrhagins exist as dimeric molecules. Both toxins possessed caseinolytic and collagenase activity although HTa was 15-36 times more potent than HTb with respect to collagenase activity. No zinc could be detected in the toxins; however, dialysis against ethylenediamine tetracetic acid (EDTA) reduced caseinolytic activity, suggesting the dependence of the latter on other metal ions. HTa and HTb had a marked effect on the intrinsic cascade coagulation mechanism (factors IX, XI and XII) but no effect on the final common coagulation pathway (factor X and prothrombin). Light and electron microscopical studies demonstrated that both HTa and HTb caused organ-specific lesions, with the lungs, diaphragm and body wall muscle being most affected. HTa caused widespread haemorrhage whilst HTb caused discrete focal lesions near the site of injection and elsewhere. However, both toxins ap...Continue Reading
References
Aug 8, 1978·Biochemistry·J B Bjarnason, A T Tu
Jan 1, 1978·Toxicon : Official Journal of the International Society on Toxinology·M Ovadia
Aug 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·H I RosenbergA Bdolah
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·A S KamigutiR A Hutton
Nov 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·D A Warrell
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·J G SotoS A Minton
Feb 1, 1974·British Journal of Haematology·N A Marsh, B C Whaler
Oct 15, 1969·Analytical Biochemistry·G L Moore
Aug 15, 1970·Nature·U K Laemmli
Sep 1, 1965·The Biochemical Journal·P Andrews
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·N A Marsh, B C Whaler
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·A CeveseB C Whaler
Dec 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·G BorkowM Ovadia
Jul 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·M T AssakuraF R Mandelbaum
Aug 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·L MoreiraJ M Gutiérrez
Dec 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·D ChakrabartyS C Lahiri
Sep 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·G BorkowM Ovadia
Jan 1, 1993·Transactions of the Royal Society of Tropical Medicine and Hygiene·T McNallyS J Machin
Mar 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·E K Johnson, C L Ownby
Apr 1, 1955·Biochimica Et Biophysica Acta·G W SCHWERT, Y TAKENAKA
Dec 1, 1959·Canadian Journal of Biochemistry and Physiology·B C HUMMEL
Citations
Jan 1, 1997·Life Sciences·N MarshG Losano
Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Adrijana LeonardiIgor Krizaj
Apr 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·N A MarshE A Bennett
Nov 22, 2000·Biochimie·J M Gutiérrez, A Rucavado
Nov 16, 2004·Journal of Cellular Biochemistry·Cecilia DíazJosé María Gutiérrez
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Neville Marsh, Vaughan Williams
Mar 16, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Neville MarshMichael Touger
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·José María GutiérrezCecilia Díaz