Sep 1, 1991

Isolation and Characterization of 51 embryo-specific Mutations of Maize

The Plant Cell
J. K. Clark, W. F. Sheridan

Abstract

A plant embryo consists of an embryonic axis, which eventually grows into the adult body, and one or two nutritive structures, the cotyledons. In the grasses embryo morphogenesis can be divided into three periods: during the first the embryo is regionalized into an embryo proper and suspensor, during the second the embryonic axis is established, and during the third vegetative structures are elaborated. Maize, with its well-characterized embryo-genesis, powerful genetics, and transposon tagging stocks, offers an attractive system for mutational analysis of these events. We have isolated 51 embryo-specific (emb) mutations from active Robertson's Mutator maize stocks. These are single-gene recessive lethals that represent at least 45 independent mutation events. Each of the 25 mutations was located to a chromosome arm using a B-A translocation set that uncovers approximately 40% of the genome; the same test failed to locate 20 others. The embryo phenotype of 27 mutations was characterized by examining mature mutant embryos in fresh dissection: the various emb mutations differ in phenotype and each is consistent in its expression. All 27 mutations result in retarded embryos that are morphologically abnormal. Nine mutants are block...Continue Reading

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Mentioned in this Paper

Embryo
Embryo Structure
Entire Embryo
Arm of Chromosome
Zea mays
Neoplasms, Germ Cell and Embryonal
Genes, Jumping
Morphogenesis
Mutant
Intracellular Translocation

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