Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation

International Journal of Environmental Research and Public Health
Shiyue LiuJuan Li

Abstract

Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h)-1, Km 66.68 ± 8.95 mg L-1, and Ki 43.16 ± 5.92 mg L-1, with an R² of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L-1 could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes.

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Feb 5, 2005·Journal of Food Protection·O Guillaume-GentilH Joosten
Aug 30, 2008·Applied Microbiology and Biotechnology·Ping ZengJoo-Hwa Tay
Jan 29, 2011·Archives of Oral Biology·Guo-Xian WeiChristine D Wu
Apr 23, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mohamed S OthmanAhmed E Abdel Moneim
May 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert D WojtyczkaDanuta Idzik
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Oct 14, 2017·Journal of Asian Natural Products Research·Wei LiHong-Xiang Lou

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Citations

Dec 8, 2020·Journal of Environmental Management·Monika SharmaRam Krishan Negi

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Datasets Mentioned

BETA
MH301295

Methods Mentioned

BETA
light microscopy

Software Mentioned

ORIGIN
CLUSTAL W
BLAST

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