PMID: 3214176Dec 1, 1988Paper

Isolation and characterization of a basic carboxypeptidase from human seminal plasma

Archives of Biochemistry and Biophysics
R A SkidgelR M Davis

Abstract

A carboxypeptidase which cleaves the C-terminal arginine or lysine from peptides was purified by a two-step procedure; gel filtration on Sephacryl S-300 and affinity chromatography on arginine-Sepharose. The activity increased 280% after the first step, indicating the removal of an inhibitor from the crude starting material. The activity in the crude seminal plasma eluted from the Sephacryl S-300 column with an apparent Mr 98,000 and after purification with an Mr 67,000, indicating that it binds to another protein in the crude seminal plasma. When analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, a single band at Mr 53,000 was seen which was converted to two smaller bands (Mr 32,000 and/or 26,000) after reduction. The seminal plasma carboxypeptidase has a neutral pH optimum, is inhibited by o-phenanthroline and by the inhibitor of carboxypeptidase B-type enzymes, 2-mercaptomethyl-3-guanidinoethylthiopropanoic acid, and can be activated by cobalt. The purified enzyme has a high specific activity (67.8 mumol/min/mg) with the ester substrate benzoyl (Bz)-Gly-argininic acid and readily cleaves Bz-Ala-Lys, Bz-Gly-Arg, and Bz-Gly-Lys. It also hydrolyzes biologically active peptides such as brad...Continue Reading

References

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Sep 1, 1985·Biological Chemistry Hoppe-Seyler·E FinkK Shimamoto
Apr 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W Hofmann, R Geiger
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Citations

Dec 25, 2013·International Journal of Biological Macromolecules·Sanjay KumarSavita Yadav
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·A NagaeE G Erdös
Feb 10, 2009·BMC Molecular Biology·Alessander O GuimarãesJoão B Pesquero
Aug 1, 1988·Trends in Pharmacological Sciences·R A Skidgel
Oct 30, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kathleen DeiterenAnne Marie Lambeir
Jan 9, 2007·Biochimica Et Biophysica Acta·Kathleen DeiterenAnne-Marie Lambeir
May 15, 2010·Biochimie·Daniel FernándezFrancesc X Avilés
Oct 29, 2002·Chemistry & Biology·Eliada LazouraHidechika Okada
Feb 9, 2019·Protein and Peptide Letters·Archana KumarKamini A Rao
Apr 18, 1997·The Journal of Biological Chemistry·L Song, L D Fricker
Dec 1, 1992·Journal of Neurochemistry·A NagaeE G Erdös
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A KuoppalaJ O Kokkonen
May 25, 1993·Biochemical Pharmacology·A BoettgerG Heder

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