PMID: 9555025May 16, 1998Paper

Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc-33, a C. elegans gene implicated in axonal outgrowth and guidance

Brain Research. Molecular Brain Research
T KamataT Copeland

Abstract

We have cloned the cDNA encoding bovine CRMP-2 from bovine brains. A full length cDNA encoding bovine CRMP-2 was isolated and sequenced. The deduced amino acid sequence reveals that the gene encodes a protein of 572 amino acids and is highly homologous to Caenorhabditis elegans unc-33, which controls the guidance and outgrowth of neuronal axons. The CRMP-2 transcript was present in bovine brains but not non-neural tissues, and its protein product existed in both soluble and membrane-bound forms. The expression of CRMP-2 protein and mRNA was upregulated during neuronal differentiation of rat PC12 cells. Immunoprecipitation of PC12 cell extracts shows that CRMP-2 was co-immunoprecipitated with a 190 kDa protein (p190). Both CRMP-2 and p190 were phosphorylated on serine residues in vivo and in vitro in a kinase assay of CRMP-2 immune complexes. Immunocytochemistry shows that CRMP-2 was exclusively localized in both the central and peripheral nervous systems in mouse embryos and detectable in the adult brain although the level of CRMP-2 decreased. The protein was expressed in the axon, dendrite, and cytoplasm of postmitotic neurons and in the cytoplasm of oligodendrocytes and astrocytes. The CRMP-2 gene maps to the region of mouse ...Continue Reading

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Citations

Jul 24, 1998·Brain Research. Molecular Brain Research·T KamataR H Xu
Feb 14, 2002·Trends in Cardiovascular Medicine·Gera NeufeldYael Herzog
Nov 29, 2001·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·M R ThambisettyN J Newman
Dec 14, 1999·The European Journal of Neuroscience·J HonnoratJ C Antoine
Jan 26, 2007·Journal of Neurochemistry·Pål StenmarkPetri Kursula
Jun 23, 2004·Biochemical and Biophysical Research Communications·Yoshinori KodamaMasahide Takahashi
Apr 25, 2006·Brain Research·Lieselotte CnopsLutgarde Arckens
Dec 7, 2007·The European Journal of Neuroscience·Ekatherina ToumaTatsuro Koike
Jun 30, 2000·Japanese Journal of Pharmacology·Y GoshimaT Kimura
Mar 23, 2011·Free Radical Research·Koji FukuiShiro Urano
Dec 29, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Qi YaoXue Zhong Liu
Dec 14, 2001·The Journal of Biological Chemistry·Sukmook LeeSung Ho Ryu
Sep 19, 2001·The Journal of Biological Chemistry·K MiuraT Kamata
Mar 28, 2019·Molecular Neurobiology·Aubin MoutalRajesh Khanna
Nov 2, 2005·Molecular and Cellular Biology·Yoji KawanoKozo Kaibuchi
Apr 16, 2018·Neuroscience·Aubin MoutalRajesh Khanna
Sep 10, 1999·La Revue de médecine interne·J HonnoratJ C Antoine
Jun 10, 2019·Journal of Neuroimmunology·Valentyna SavchenkoDouglas L Feinstein
May 16, 2000·Science·R S Nowakowski, N L Hayes

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