Isolation and characterization of a catalase gene "HuCAT3" from pitaya (Hylocereus undatus) and its expression under abiotic stress

Gene
Qiong NieYong-Qiang Cai

Abstract

Abiotic stresses usually cause H2O2 accumulation, with harmful effects, in plants. Catalase may play a key protective role in plant cells by detoxifying this excess H2O2. Pitaya (Hylocereus undatus) shows broad ecological adaptation due to its high tolerance to abiotic stresses, e.g. drought, heat and poor soil. However, involvement of the pitaya catalase gene (HuCAT) in tolerance to abiotic stresses is unknown. In the present study, a full-length HuCAT3 cDNA (1870 bp) was isolated from pitaya based on our previous microarray data and RACE method. The cDNA sequence and deduced amino acid sequence shared 73-77% and 75-80% identity with other plant catalases, respectively. HuCAT3 contains conserved catalase family domain and catalytic sites. Pairwise comparison and phylogenetic analysis indicated that HuCAT3 is most similar to Eriobotrya japonica CAT, followed by Dimocarpus longan CAT and Nicotiana tabacum CAT1. Expression profile analysis demonstrated that HuCAT3 is mainly expressed in green cotyledons and mature stems, and was regulated by H2O2, drought, cold and salt stress, whereas, its expression patterns and maximum expression levels varied with stress types. HuCAT activity increased as exposure to the tested stresses, and ...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M G RedinbaughJ G Scandalios
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·H WillekensW Van Camp
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G NoctorC H Foyer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Janine KönigKarl-Josef Dietz
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Jun 18, 2004·The Plant Journal : for Cell and Molecular Biology·Steven VandenabeeleFrank Van Breusegem
Oct 12, 2004·Journal of Experimental Botany·M M Chaves, M M Oliveira
Dec 18, 2004·Journal of Plant Physiology·Attipalli Ramachandra ReddyMunusamy Vivekanandan
Nov 4, 2006·Plant, Cell & Environment·Petra ZimmermannUlrike Zentgraf
Jun 9, 2007·Molecular Plant-microbe Interactions : MPMI·Zenaida V MagbanuaDawn S Luthe
Sep 11, 2007·Colloids and Surfaces. B, Biointerfaces·Bilian ChenLingling Huang
Nov 20, 2008·Journal of Integrative Plant Biology·Yan-Yan DuChun-Peng Song
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Sep 30, 2010·Journal of Experimental Botany·Amna MhamdiGraham Noctor
Jan 1, 2009·The Arabidopsis Book·Navneet KaurJianping Hu

❮ Previous
Next ❯

Citations

Aug 24, 2016·Environmental Science and Pollution Research International·Naser A AnjumNarendra Tuteja
Nov 8, 2017·International Journal of Molecular Sciences·Xuan DongDe-Gang Zhao
Jun 24, 2017·BioMed Research International·Bin YongHuanhuan Shao
Sep 12, 2018·International Journal of Molecular Sciences·Tingting SunYachun Su
Jul 2, 2020·International Journal of Molecular Sciences·Yujie QuKuaifei Xia
Nov 19, 2015·Frontiers in Plant Science·Dong CiDeqiang Zhang
May 28, 2019·FEBS Open Bio·Quandong NongKuaifei Xia
Jun 9, 2020·Redox Biology·José M PalmaFrancisco J Corpas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.