Isolation and characterization of a cDNA encoding a Xenopus immunoglobulin binding protein, BiP (grp78)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
D MiskovicJohn J Heikkila

Abstract

We have isolated a full-length cDNA clone encoding a Xenopus laevis immunoglobulin binding protein (BiP; also called glucose-regulated protein or grp78). The Bip cDNA sequence includes an open reading frame of 1,965 bp encoding a 655 amino acid protein with an N-terminal hydrophobic leader sequence and a C-terminal KDEL tetrapeptide which has been found in other lumenal proteins of the endoplasmic reticulum. The 3' untranslated region contains a polyadenylation and an adenylation control element (ACE) as well as a putative mRNA instability sequence. The Xenopus BiP amino acid sequence displayed high identity with BiP from other vertebrates including chicken (91.3%), rat (90.7%), and human (89.9%). Northern hybridization analysis demonstrated that BiP mRNA was present constitutively in the Xenopus A6 kidney epithelial cell line and that BiP mRNA levels could be enhanced by treatment of the cells with galactose-free media, 2-deoxyglucose, 2-deoxygalactose, glucosamine, tunicamycin, heat shock, dithiothreitol, and the calcium ionophore, A23187. Finally, while BiP mRNA was detected in all of the adult tissues examined, the relative level of BiP mRNA differed dramatically between organs. For example, relatively high levels of BiP mR...Continue Reading

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Citations

Nov 28, 2013·Genome Biology and Evolution·Jacek KominekBarry L Williams
Apr 5, 2012·Experimental Diabetes Research·Daniel R Beriault, Geoff H Werstuck
Feb 9, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·John J Heikkila
Oct 28, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Cody S Shirriff, John J Heikkila
Jan 3, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amanda M Hamilton, John J Heikkila
Jul 19, 2006·Canadian Journal of Physiology and Pharmacology·Lindsie A RobertsonGeoff H Werstuck
Sep 25, 2004·Canadian Journal of Physiology and Pharmacology·M MullerJohn J Heikkila
Jul 15, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shinya Kawabe, Yoshihiro Yokoyama

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