Isolation and characterization of a cDNA encoding a potential morphogen from the marine sponge Geodia cydonium that is conserved in higher metazoans

Proceedings. Biological Sciences
S PahlerW E Müller

Abstract

Species belonging to the lowest metazoan phylum, the sponges (Porifera), exhibit a surprisingly complex and multifaceted Bauplan (body plan). Recently, key molecules have been isolated from sponges which demonstrate that the cells of these animals are provided with characteristic metazoan adhesion and signal transduction molecules, allowing tissue formation. In order to understand which factors control the spatial organization of these cells in the sponge body plan, we screened for a cDNA encoding a soluble modulator of the behaviour of endothelial cells. A cDNA encoding a putative protein, which is highly similar to the human and mouse endothelial monocyte-activating polypeptide (EMAP) II has been isolated from a library of the marine sponge Geodia cydonium. The sponge EMAP-related polypeptide (EMAPR) has been termed EMAPR1_GC. The full-length cDNA clone, GCEMAPR1, has a size of 592 nucleotides (nt) and contains a 447 nt-long potential open reading frame; the molecular weight (MW) of the deduced amino acid sequence, 16,499 Da, is close to that of mature mammalian EMAP II (ca. 18 kDa). The sponge polypeptide is also closely related to a deduced polypeptide from the cosmid clone F58B3 isolated from Caenorhabditis elegans. A phyl...Continue Reading

References

Feb 1, 1992·Cell and Tissue Research·N SundarRajJ P Gesiotto
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 1, 1995·Die Naturwissenschaften·W E Müller
Jun 1, 1993·Acta Pathologica Japonica·M Yanagishita

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Citations

Jun 26, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W E Müller
Oct 13, 2004·The Keio Journal of Medicine·Werner E G MüllerIsabel M Müller
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H C SchröderW E Müller
Jun 15, 2000·The Journal of Biological Chemistry·P B Miarons, M Fresno

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