Isolation and characterization of a cold-induced nonculturable suppression mutant of Vibrio vulnificus

Microbiological Research
Akihisa AbeHideaki Endo

Abstract

The viable but nonculturable (VBNC) suppression mutant formed platable cells at low temperature stress after inoculation in artificial seawater (ASW). Suppression subtractive hybridization was used to identify differentially expressed genes among cDNAs of the VBNC suppression mutant and the wild-type Vibrio vulnificus strain. Glutathione S-transferase was identified as a responsive gene of the VBNC suppression mutant in our assay, and was highly expressed from the VBNC suppression mutant at low temperature stress. Culturability tests revealed that the wild-type cells were sensitive to oxidative stress in the hydrogen peroxide (H(2)O(2)) and to 1-chloro-2,4-dinitrobenzene (CDNB) compared with the VBNC suppression mutant cells. Adding glutathione showed that many wild-type V. vulnificus cells maintained culturability in cold ASW. These results suggest that non-nutritional growth inhibitors, such as peroxide that accumulates at low temperatures, influence VBNC in V. vulnificus cells.

References

Mar 1, 1989·Archives of Ophthalmology·M DiGaetanoJ G Straus
Feb 1, 1987·Diagnostic Microbiology and Infectious Disease·R C Tilton, R W Ryan
Oct 14, 1983·Science·T D Sargent, I B Dawid
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Y MoonN Murata
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z GombosN Murata
Nov 15, 1995·FEMS Microbiology Letters·J D Oliver
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Jan 3, 1997·The Journal of Biological Chemistry·W JiangM Inouye
Jan 1, 1997·Chemical Research in Toxicology·R N Armstrong
May 14, 1999·Current Opinion in Microbiology·S PhadtareM Inouye

❮ Previous
Next ❯

Citations

Apr 9, 2013·Applied and Environmental Microbiology·Hen-Wei WangHin-chung Wong
Dec 15, 2010·Applied and Environmental Microbiology·Richard J ThomasTimothy Atkins
Oct 27, 2009·Current Opinion in Microbiology·Christopher S Hayes, David A Low
Sep 9, 2009·International Journal of Food Microbiology·Chiou-Jour LaiHin-Chung Wong
Jun 25, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Rashed NoorMamoru Yamada
Jan 12, 2010·FEMS Microbiology Reviews·James D Oliver
Aug 19, 2014·Frontiers in Public Health·Thandavarayan RamamurthySumio Shinoda
Jun 12, 2014·Frontiers in Microbiology·Laam LiSebastien P Faucher
Jul 25, 2009·FEMS Microbiology Letters·Raquel del CampoCarina Gaggero
Aug 30, 2016·Nature Methods·Matthew T WeinstockDaniel G Gibson
Feb 1, 2019·Critical Reviews in Food Science and Nutrition·Jae-Hyun Yoon, Sun-Young Lee
Mar 8, 2019·Microbiology·Shravanthi S Kumar, Asit Ranjan Ghosh
Jun 12, 2018·Frontiers in Microbiology·Paula M M MartinsAlessandra A De Souza
Feb 6, 2021·World Journal of Microbiology & Biotechnology·Laurens MaertensRob Van Houdt
Dec 16, 2020·Comprehensive Reviews in Food Science and Food Safety·Kai DongXiaojun Liao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.