Isolation and characterization of a composite plasmid Rms201 mutant temperature sensitive for replication.

Journal of Bacteriology
Y IkeS Mitsuhashi

Abstract

A mutant temperature-sensitive for R-plasmid replication, Rms201ts14, was isolated from composite plasmid Rms201 after mutagenesis of P1 transducing lysate with 100 mM hydroxylamine for 40 h at 37 degrees C. When Escherichia coli ML1410(Rms201ts14)(+) was grown at temperatures between 40 and 42 degrees C in L broth, antibiotic-sensitive cells were segregated. When the incubation temperature of ML1410(Rms201ts14)(+) in L-broth was shifted to 42 from 30 degrees C, the increase in the number of antibiotic-resistant cells ceased 90 min after the temperature shift. However, the total number of cells continuously increased, and only 3% of the cells retained the plasmid at 5 h after the temperature shift to 42 degrees C. At 30 degrees C the amounts of covalently closed circular deoxyribonucleic acid per chromosome of Rms201ts14 and Rms201 were 3.8 and 6.3%, respectively. Incorporation of radioactive thymidine into the covalently closed circular deoxyribonucleic acid of Rms201ts14 did not take place at 42 degrees C, whereas radioactive thymidine was incorporated into the covalently closed circular deoxyribonucleic acid of Rms201 at a rate of 4%/chromosome even at 42 degrees C. The synthesis of plasmid covalently closed circular deoxyri...Continue Reading

References

May 1, 1979·Journal of Bacteriology·R Eichenlaub
Jan 1, 1979·Microbiology and Immunology·Y IkeS Mitsuhashi
Jul 1, 1976·Journal of Bacteriology·N TanakR H Rownd
Mar 1, 1975·Journal of Bacteriology·Y TerawakiR Nakaya
Oct 1, 1975·Journal of Bacteriology·F J KretschmerS N Cohen
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·F HeffronS Falkow
Sep 1, 1967·Journal of Bacteriology·Y TerawakiT Akiba
Oct 15, 1973·Biochemical and Biophysical Research Communications·Y M Kupersztoch, D R Helinski
Sep 1, 1973·Japanese Journal of Microbiology·T TanakaS Mitsuhashi
Jun 1, 1974·Journal of Bacteriology·C F MorrisR Rownd
Sep 1, 1974·Journal of Bacteriology·E M Lederberg, S N Cohen
Dec 1, 1974·Journal of Bacteriology·Y OdakuraS Mitsuhashi
Jun 1, 1969·Bacteriological Reviews·R P Novick
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J S Hong, B N Ames

❮ Previous
Next ❯

Citations

Aug 1, 1981·Journal of Bacteriology·Y IkeS Mitsuhashi
Mar 1, 1983·Antimicrobial Agents and Chemotherapy·H Nakazawa, S Mitsuhashi
Nov 1, 1981·Journal of Bacteriology·Y IkeS Mitsuhashi
Apr 1, 1981·Journal of Bacteriology·S IyobeS Mitsuhashi
May 1, 1982·Journal of Bacteriology·K KatsuS Mitsuhashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.