Isolation and characterization of a gene for assimilatory sulfite reductase from Arabidopsis thaliana

Gene
C BorkRüdiger Hell

Abstract

Sulfite reductase (SIR) represents a key enzyme in sulfate assimilation in higher plants. The genomic DNA sequence of the sir gene from Arabidopsis thaliana including regulatory and structural regions was isolated and characterized. The sequence of a 6 kb fragment encoding SIR revealed a coding region of 2891 basepairs (bp) that consists of eight exons separated by seven introns between 83 and 139 bp in length. The transcription start point was determined 272 bp upstream of the translation start site. Southern analysis indicates a single locus for the sir gene that gives rise to a 2.4 (kb) mRNA in leaves and in roots. The promoter region was verified by functional expression of the gusA reporter gene in transgenic A. thaliana plants and was shown to provide correct expression in root and leaf.

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Citations

Apr 7, 2010·Amino Acids·Hankuil YiJoseph M Jez
Oct 18, 2011·Bioconjugate Chemistry·Olivia M MerkelThomas Kissel
Oct 2, 2012·Biomacromolecules·Danielle S W Benoit, Molly E Boutin
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Aug 19, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H GuissoumaB A Demeneix
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May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Arthur Grossman, Hideki Takahashi
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Nov 30, 2012·Plant, Cell & Environment·Guy Hanke, Paula Mulo
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Mar 3, 2019·Journal of Experimental Botany·Joseph M Jez
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Jul 10, 1999·Plant Physiology·T Leustek, K Saito

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