Isolation and characterization of a human monoclonal antibody that recognizes epitopes shared by Pseudomonas aeruginosa immunotype 1, 3, 4, and 6 lipopolysaccharides.

Infection and Immunity
A B LangS J Cryz

Abstract

A hybridoma line secreting a human monoclonal antibody (HMAb) capable of recognizing Fisher immunotype (IT) 1, 3, 4, and 6 lipopolysaccharide (LPS) in vitro was isolated. Peripheral blood lymphocytes (PBL) were obtained from volunteers immunized with an experimental Pseudomonas aeruginosa O polysaccharide-toxin A vaccine. PBL-expressing surface antibodies able to bind to P. aeruginosa LPS were isolated by adsorption onto LPS-coated plastic wells. Such PBL were transformed with Epstein-Barr virus. Lymphoblastoid cell lines secreting anti-P. aeruginosa LPS antibodies were identified by an enzyme-linked immunosorbent assay and fused to the F3B6 heteromyeloma line. A hybridoma line producing a HMAb (2-8AH79) able to bind IT-1, IT-3, IT-4, and IT-6 LPS was identified by an enzyme-linked immunosorbent assay. This HMAb was found to bind to the O-polysaccharide regions of IT-1, IT-3, IT-4, and IT-6 LPS, as determined by immunoblotting. By using an immunofluorescence microscopy assay, the cell surfaces of IT-3 and IT-4 bacteria were strongly stained by HMAb 2-8AH79, whereas those of IT-1 and IT-6 bacteria were weakly stained. This HMAb was found to promote the uptake and killing of IT-3 and IT-4 bacteria, but not IT-1 or IT-6 organisms,...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1987·Antibiotics and Chemotherapy·S J CryzR Germanier
Nov 1, 1974·The Journal of Infectious Diseases·J WesleyM W Fisher
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Nov 1, 1983·Reviews of Infectious Diseases·S G Wilkinson
Jan 1, 1982·The Journal of Infectious Diseases·C C SandersW E Sanders
Mar 1, 1983·Reviews of Infectious Diseases·G P BodeyL Jadeja
May 1, 1984·Infection and Immunity·S J CryzR Germanier
Feb 1, 1982·The Journal of Infectious Diseases·G B Pier, D M Thomas
Dec 1, 1981·The Journal of Infectious Diseases·J E Pennington, E Menkes

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Citations

Nov 1, 1992·Infection and Immunity·G B PierE Neuger

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