Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
S I KimT H Tai

Abstract

Using a forward genetics approach, we isolated two independent low phytic acid (lpa) rice mutants, N15-186 and N15-375. Both mutants are caused by single gene, recessive non-lethal mutations, which result in approximately 75% (N15-186) and 43% (N15-375) reductions in seed phytic acid (inositol hexakisphosphate). High-performance liquid chromatography and GC-MS analysis of seed extracts from N15-186 indicated that, in addition to phytic acid, inositol monophosphate was significantly reduced whereas inorganic phosphorus and myo-inositol were greatly increased when compared with wild-type. The changes observed in N15-186 resemble those previously described for the maize lpa3 mutant. Analysis of N15-375 revealed changes similar to those observed in previously characterized rice lpa1 mutants (i.e. significant reduction in phytic acid and corresponding increase in inorganic phosphorus with little or no change in inositol phosphate intermediates or myo-inositol). Further genetic analysis of the N15-186 mutant indicated that the mutation, designated lpa N15-186, was located in a region on chromosome 3 between the microsatellite markers RM15875 and RM15907. The rice orthologue of maize lpa3, which encodes a myo-inositol kinase, is in th...Continue Reading

References

May 2, 2001·Nature Reviews. Molecular Cell Biology·R F Irvine, M J Schell
Mar 7, 2002·The Journal of Nutrition·Victor Raboy
Mar 22, 2002·Trends in Plant Science·Henrik Brinch-PedersenPreben Bach Holm
Sep 13, 2002·The Journal of Biological Chemistry·Jill Stevenson-PaulikJohn D York
Aug 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Fouad Lemtiri-ChliehCharles A Brearley
Oct 3, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R PiluE Nielsen
May 28, 2005·The Plant Journal : for Cell and Molecular Biology·Jinrui ShiTeresa Harp
Jun 9, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Cynthia B Andaya, Thomas H Tai
Aug 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jill Stevenson-PaulikJohn D York
Jun 20, 2006·Biochimica Et Biophysica Acta·John D York
Jan 16, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Qing-Long LiuQing-Yao Shu
Apr 13, 2007·BMC Plant Biology·Bradley J TillLuca Comai
Aug 10, 2007·Nature Biotechnology·Victor Raboy
Aug 19, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Feng-Jie YuanQing-Yao Shu
Dec 7, 2007·Journal of Agricultural and Food Chemistry·Thomas FrankKarl-Heinz Engel
Jun 21, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S I KimT H Tai

❮ Previous
Next ❯

Citations

Jun 27, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Feng-Jie YuanQing-Yao Shu
Sep 18, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hai-Jun ZhaoQing-Yao Shu
Jan 19, 2011·Functional & Integrative Genomics·Hongxia YeChengdao Li
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sichul LeeGynheung An
Mar 22, 2014·Transgenic Research·Wen-Xu LiQing-Yao Shu
Jan 1, 2015·Plants·Francesca Sparvoli, Eleonora Cominelli
May 23, 2012·Plant Science : an International Journal of Experimental Plant Biology·Raul Antonio SperottoJanette Palma Fett
Jan 29, 2014·Plant Science : an International Journal of Experimental Plant Biology·Koya Matsuno, Tatsuhito Fujimura
Oct 30, 2015·Critical Reviews in Food Science and Nutrition·Deep Shikha BirlaPawan K Jaiwal
Feb 9, 2017·Frontiers in Plant Science·A Vinoth, R Ravindhran
Jun 13, 2017·Journal of Agricultural and Food Chemistry·Chuan TongJinsong Bao
Mar 20, 2010·Biochemical Society Transactions·Søren K RasmussenAnna Maria Torp
Dec 31, 2019·International Journal of Biological Macromolecules·Mariana N CoelhoSônia S Costa
May 27, 2021·Critical Reviews in Food Science and Nutrition·Deepak KasoteAhmed Regina
May 16, 2021·Plant Physiology and Biochemistry : PPB·Vinicius Martins SilvaAndré Rodrigues Dos Reis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.