Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B

Toxicon : Official Journal of the International Society on Toxinology
Gi-Hyeok YangHyun Ho Jung

Abstract

Botulinum neurotoxins (BoNTs), the causative agents for life-threatening human disease botulism, have been recognized as biological warfare agents. In this study, a neutralizing mouse monoclonal antibody against botulinum neurotoxin serotype B (BoNT/B), named BTBH-N1, was developed from mice immunized with BoNT/B toxoid without non-toxic components, which are generally associated with the toxin. Western blot analysis, using recombinant toxin fragments containing light (L), N-terminal half of heavy (HN) and C-terminal half of heavy chains, indicated that BTBH-N1 recognizes linear epitopes located on the HN domain. An in vivo neutralization assay with mice, was conducted to characterize the neutralization capacity of the BTBH-N1. Only 10 microg of BTBH-N1 completely neutralized 20 units (1 unit = one 50% lethal dose) of BoNT/B. Even though the Mab (up to 100 microg) failed to protect mice challenged with 100 units, it significantly prolonged the time to death in a dose dependent manner. BTBH-N1, the first neutralizing antibody against BoNT/B, could be further developed as effective biological therapeutics for preventing and treating botulism, as well as other diseases caused by BoNT/B.

References

Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Jan 1, 1982·Pharmacology & Therapeutics·G Sakaguchi
Mar 20, 1980·Biochimica Et Biophysica Acta·M KitamuraY Nagai
Sep 1, 1994·Toxicology and Applied Pharmacology·L B PearceR D MacCallum
Aug 1, 1995·Protein Science : a Publication of the Protein Society·M Oblatt-MontalM Montal
Nov 1, 1995·Quarterly Reviews of Biophysics·C Montecucco, G Schiavo
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MatteoliC Montecucco
Mar 7, 2001·JAMA : the Journal of the American Medical Association·S S ArnonUNKNOWN Working Group on Civilian Biodefense
Sep 13, 2001·Infection and Immunity·B P MullaneyJ D Marks
Jun 4, 2002·Nature Biotechnology·Jennifer A MaynardGeorge Georgiou
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·A NowakowskiJ D Marks
Dec 3, 2002·Nature Structural Biology·Lilia K Koriazova, Mauricio Montal

❮ Previous
Next ❯

Citations

Dec 12, 2007·Infection and Immunity·Michael R BaldwinJoseph T Barbieri
May 26, 2009·Applied Biochemistry and Biotechnology·Wei-Gang HuLes P Nagata
Feb 7, 2008·Expert Review of Vaccines·Laura M ZarebskiAlessandro Sette
Aug 28, 2012·Toxicon : Official Journal of the International Society on Toxinology·Alexey ZdanovskyMaria Zdanovskaia
Nov 30, 2006·Immunology and Cell Biology·Yi-Hua ZhouSuzanne U Emerson
Jun 12, 2013·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Daniel E Forman, Richert E Goyette
Jul 24, 2012·Toxins·Siu-Kei Chow, Arturo Casadevall

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A NowakowskiJames D Marks
Pharmacology & Therapeutics
G Sakaguchi
JAMA : the Journal of the American Medical Association
S S ArnonWorking Group on Civilian Biodefense
© 2022 Meta ULC. All rights reserved