PMID: 3922410Apr 23, 1985

Isolation and characterization of a new endo-beta-galactosidase from Diplococcus pneumoniae

Biochemistry
M N Fukuda

Abstract

An endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of R----GlcNAc (or GalNAc) beta 1----3Gal beta 1----4GlcNAc (or Glc), was isolated from the culture supernatant of Diplococcus pneumoniae. The enzyme, named endo-beta-galactosidase DII, hydrolyzed linear N-acetyllactosamine repeating structures in glycolipids and glycopeptides to release oligosaccharides. The specificity of endo-beta-galactosidase DII is the same as that of Escherichia freundii endo-beta-galactosidase as far as described above, but the following differences between these two enzymes were found: Branched lactosaminyl glycolipids and H-antigenic glycolipids were resistant to endo-beta-galactosidase DII, even when linear structure was present at the inner part. Throughout the enzymic hydrolysis, endo-beta-galactosidase DII released mostly small oligosaccharides (tetra-, tri-, and disaccharides) from substrates, suggesting that the enzyme split off the oligosaccharides stepwise from the nonreducing terminal. Lactosaminoglycans were partially hydrolyzed by endo-beta-galactosidase DII to produce small oligosaccharides as the major product and residual glycopeptides. The residual glycopeptides were readily hydrolyzed by E. freundii en...Continue Reading

References

Jan 1, 1984·The Japanese Journal of Physiology·Y Fukuda
Apr 1, 1961·British Journal of Haematology·W L MARSH

Citations

Jan 1, 1987·Developmental Biology·A DuttD D Carson
Nov 15, 1987·Archives of Biochemistry and Biophysics·H T Sojar, O P Bahl
Feb 12, 2008·Current Protocols in Molecular Biology·M Fukuda

Related Concepts

Keratan-sulfate endo-1,4-b-galactosidase
Anion Exchange Protein 1, Erythrocyte
Lactogest
Carbohydrate Linkage
High Pressure Liquid Chromatography Procedure
Thin Layer Chromatography
Erythrocytes
Galactosidase
Glycolipids
Exoglycosidases

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