Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana

Journal of Applied Microbiology
N AyyaduraiN Sakthivel

Abstract

Isolation and characterization of a bacterial isolate (strain FP10) from banana rhizosphere with innate potential as fungal antagonist and microbial adjuvant in micropropagation of banana. Bacterium FP10 was isolated from the banana rhizosphere and identified as Pseudomonas aeruginosa based on phenotypic, biochemical traits and sequence homology of partial 622-bp fragment of 16S ribosomal DNA (rDNA) amplicon, with the ribosomal database sequences. Strain FP10 displayed antibiosis towards fungi causing wilt and root necrosis diseases of banana. Production of plant growth hormone, indole-3-acetic acid (IAA), siderophores and phosphate-solubilizing enzyme in FP10 was determined. Strain FP10 tested negative for hydrogen cyanide, cellulase and pectinase, the deleterious traits for plant growth. Screening of antibiotic genes was carried out by polymerase chain reaction using gene-specific primers. Amplification of a 745-bp DNA fragment confirmed the presence of phlD, which is a key gene involved in the biosynthesis of 2,4-diacetylphloroglucinol (DAPG) in FP10. The antibiotic produced by FP10 was confirmed as DAPG using thin layer chromatography, high performance liquid chromatography and Fourier transform infrared and tested for fung...Continue Reading

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Citations

Mar 19, 2008·Microbial Ecology·Popavath Ravindra NaikNatarajan Sakthivel
Nov 16, 2010·Canadian Journal of Microbiology·Geoffrey G Bradley, Zamir K Punja
Jul 11, 2014·Ecotoxicology and Environmental Safety·Ivy MallickSamir Kumar Mukherjee
Aug 19, 2015·Apoptosis : an International Journal on Programmed Cell Death·Vijay Kumar VeenaNatarajan Sakthivel
Jun 22, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Syed Baker, Sreedharamurthy Satish
Apr 27, 2019·Frontiers in Microbiology·Giovanni BubiciJesús Mercado-Blanco

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