PMID: 9178809May 16, 1997Paper

Isolation and characterization of a novel gene from human glioblastoma multiforme tumor tissue

International Journal of Cancer. Journal International Du Cancer
A SehgalG P Murphy

Abstract

Using the technique of DD-PCR (differential display-polymerase chain reaction) we isolated a novel gene (D2-2) that is overexpressed in glioblastoma multiforme tissue (GMT) as compared to normal brain tissue (NBT). D2-2 is also highly expressed in recurrent glioma, colon tumor metastatic to brain, breast tumors, prostate tumors and a prostate tumor cell line (LNCaP). Northern blot analysis showed that D2-2 is highly expressed in several tumor cell lines (MOLT lymphoblastic leukemia, SW480 colorectal adrenocarcinoma, A549 lung carcinoma, HL-60 promyelocytic leukemia, S3 HeLa cells, K-562 chronic myelogeneous leukemia and G361 melanoma) as compared to NBT. Additionally, D2-2 is very highly expressed in cell lines derived from glioblastomas, grade IV astrocytomas, normal human fetal astrocytes (NHFA) and glioma. D2-2 is moderately expressed in neuroblastoma, neuroectodermal and medulloblastoma tumor cell lines. D2-2 expression is localized to the frontal lobe, occipital lobe and the cerebellum in the normal brain. Normal tissues such as thyroid, stomach, adrenal cortex, small intestine and pancreas show high expression of D2-2. We also show that D2-2 is expressed 28-fold higher in fetal brain (20 weeks) than in adult brain. Sequen...Continue Reading

References

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Citations

May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·K OkinoT Takeshita
Jan 4, 1998·Seminars in Surgical Oncology·A Sehgal
May 19, 2006·International Journal of Environmental Research and Public Health·Jacqueline J StevensLai-Man Chan
May 20, 1998·International Journal of Cancer. Journal International Du Cancer·A SehgalG P Murphy
Dec 31, 1998·Current Opinion in Neurology·J L Darling, T J Warr
Jan 9, 1999·Journal of Surgical Oncology·A SehgalG P Murphy
Jun 15, 2010·Genes, Chromosomes & Cancer·Casey M WrightKwun M Fong
May 17, 2017·International Journal of Cancer. Journal International Du Cancer·Kaipeng XieZhibin Hu

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