Isolation and characterization of a novel low molecular weight protein involved in intra-Golgi traffic.

The Journal of Biological Chemistry
A Legesse-MillerZvulun Elazar

Abstract

Analysis of the cytosolic requirements for in vitro intra-Golgi transport led to the characterization of three proteins: N-ethylmaleimide-sensitive fusion protein (NSF), soluble NSF attachment protein (SNAP), and p115, all involved in the docking and fusion of transport vesicles to their target membranes. In the course of determining the minimal cytosolic requirements for intra-Golgi transport in vitro, we identified three additional factors that are sufficient to replace crude cytosol. We describe here the purification and characterization of one of these factors, a novel 16-kDa protein, p16, an essential factor for intra-Golgi protein transport. Based on transport activity, this purification procedure resulted in approximately 1,400-fold enrichment of p16 to apparent homogeneity. The activity of p16 could be observed in the absence of vesicle formation, suggesting that it may participate in the docking and fusion processes.

References

Jan 1, 1992·Methods in Enzymology·M R Block, J E Rothman
Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
Jan 30, 1992·Nature·J E Rothman, L Orci
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R BlockJ E Rothman
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K SappersteinM G Waters
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BarrosoE Sztul
Nov 3, 1994·Nature·J E Rothman
Feb 1, 1994·The Journal of Cell Biology·Z ElazarJ E Rothman
Dec 3, 1993·Cell·J OstermannJ E Rothman
Mar 1, 1996·The Journal of Cell Biology·S K SappersteinM G Waters
Jan 1, 1996·Annual Review of Cell and Developmental Biology·S R Pfeffer

❮ Previous
Next ❯

Citations

Feb 13, 2001·Microscopy Research and Technique·M Thumm
Mar 24, 2004·Journal of Structural Biology·Sidney W Whiteheart, Elena A Matveeva
Jun 28, 2002·Brain Research. Brain Research Reviews·Matthias Kneussel
Jan 31, 2002·EMBO Reports·Vassiliy N BavroWinfried Weissenhorn
Aug 27, 2011·Genome Biology·Tomer ShpilkaZvulun Elazar
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M KneusselH Betz
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Apr 3, 2007·FEBS Letters·Chunxia ZhaoSidney W Whiteheart
Jun 15, 2011·Experimental Cell Research·Wenbin ZhongDaoguang Yan
Apr 13, 2010·Biochemical and Biophysical Research Communications·Melanie SchwartenDieter Willbold
Jul 22, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kenji SugawaraFuyuhiko Inagaki
Nov 7, 2006·Journal of Neurochemistry·Zi-Wei Chen, Richard W Olsen
Jan 1, 2003·Biochemical and Biophysical Research Communications·Isei TanidaEiki Kominami
Nov 26, 2013·Biochemical and Biophysical Research Communications·Suzy Choi, Hyun Jin Kim
Aug 14, 2003·Biochimica Et Biophysica Acta·Zvulun ElazarHagai Shorer
May 9, 2002·FEBS Letters·Frank GreenCaroline A Enns
Apr 7, 2004·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Joseph E Coyle, Dimitar B Nikolov
Dec 11, 2002·The Journal of Biological Chemistry·Ruth Scherz-ShouvalZvulun Elazar
Apr 29, 2005·The Journal of Biological Chemistry·Takahide KounoKeiichi Kawano
Jan 25, 2006·The Journal of Biological Chemistry·Chongguang ChenLee-Yuan Liu-Chen
Jan 22, 2020·Protein Science : a Publication of the Protein Society·Saki TaniguchiJoji Mima
Sep 8, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marco B E SchaafKasper M A Rouschop
Jun 6, 2000·The Journal of Biological Chemistry·A Legesse-MillerZ Elazar
Jun 29, 2000·The Journal of Biological Chemistry·A Porat, Z Elazar
Apr 13, 2004·Oncogene·Devrim Gozuacik, Adi Kimchi
Aug 28, 2020·Journal of Cell Science·Franziska EckChristian Behrends
May 9, 2000·The Journal of Biological Chemistry·A PoratZ Elazar
May 8, 2020·Cell Discovery·Sascha Martens, Dorotea Fracchiolla
Sep 27, 2005·The Journal of Biological Chemistry·Kenji SugawaraFuyuhiko Inagaki
Dec 31, 2020·International Journal of Molecular Sciences·Julia L SanwaldDieter Willbold
Dec 19, 2013·Journal of Cell Science·Philipp WildIvan Dikic
May 6, 2021·Cell Discovery·Sascha Martens, Dorotea Fracchiolla

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.