Isolation and characterization of a novel phage lysin active against Paenibacillus larvae, a honeybee pathogen

Bacteriophage
Lucy LeBlancPenny S Amy

Abstract

Paenibacillus larvae is the causative agent of American foulbrood (AFB) disease which affects early larval stages during honeybee development. Due to its virulence, transmissibility, capacity to develop antibiotic resistance, and the inherent resilience of its endospores, Paenibacillus larvae is extremely difficult to eradicate from infected hives which often must be burned. AFB contributes to the worldwide decline of honeybee populations, which are crucial for pollination and the food supply. We have isolated a novel bacteriophage lysin, PlyPalA, from the genome of a novel Paenibacillus larvae bacteriophage originally extracted from an environmental sample. PlyPalA has an N-terminal N-acetylmuramoyl-L-alanine amidase catalytic domain and possesses lytic activity against infectious strains of Paenibacillus larvae without harming commensal bacteria known to compose the honeybee larval microbiota. A single dose of PlyPalA rescued 75% of larvae infected with endospores, showing that it represents a powerful tool for future treatment of AFB. This represents the first time that lysins have been tested for therapeutic use in invertebrates.

References

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Citations

Oct 16, 2016·Bacteriophage·Casey StamereilersPhilippos K Tsourkas
Aug 12, 2016·Biochemistry·Meenakshi SharmaKrishna Mohan Poluri
Jul 22, 2018·Viruses·Casey StamereilersPhilippos K Tsourkas
Aug 28, 2020·Frontiers in Microbiology·Ewa Jończyk-MatysiakAndrzej Górski
Feb 24, 2019·Scientific Reports·Sílvio B SantosJoana Azeredo
Mar 1, 2020·Microbial Genomics·Philippos K Tsourkas

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Datasets Mentioned

BETA
KT167538

Methods Mentioned

BETA
PCR
fluorescence assay
electrophoresis

Software Mentioned

CLC Main Workbench
PlyPalA
GraphPad
BLASTP
DNA Master
Prism
MiSeq
Geneious

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