PMID: 11606203Oct 19, 2001Paper

Isolation and characterization of a novel type of neurotoxic peptide from the venom of the South African scorpion Parabuthus transvaalicus (Buthidae)

European Journal of Biochemistry
Bora InceogluBruce D Hammock

Abstract

The venom of the South African scorpion Parabuthus transvaalicus was characterized using a combination of mass spectrometry and RP-HPLC separation and bioassays. The crude venom was initially separated into 10 fractions. A novel, moderately toxic but very high abundance peptide (birtoxin) of 58 amino-acid residues was isolated, identified and characterized. Each purification step was followed by bioassays and mass spectroscopy. First a C4 RP-HPLC column was used, then a C18 RP Microbore column purification resulted in > 95% purity in the case of birtoxin from a starting material of 230 microg of crude venom. About 12-14% of the D214 absorbance of the total venom as observed after the first chromatography step was composed of birtoxin. This peptide was lethal to mice at low microgram quantities and it induced serious symptoms including tremors, which lasted up to 24 h post injection, at submicrogram amounts. At least seven other fractions that showed different activities including one fraction with specificity against blowfly larvae were identified. Identification of potent components is an important step in designing and obtaining effective anti-venom. Antibodies raised against the critical toxic components have the potential t...Continue Reading

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Citations

Jun 21, 2005·Biochemical and Biophysical Research Communications·Marie-France Martin-EauclairePierre E Bougis
Oct 8, 2003·Toxicon : Official Journal of the International Society on Toxinology·Oren Froy, Michael Gurevitz
Jun 6, 2003·European Journal of Biochemistry·Dalia GordonMichael Gurevitz
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bora InceogluBruce D Hammock
Mar 19, 2014·Toxicon : Official Journal of the International Society on Toxinology·Bea G MilleJan Tytgat
Nov 26, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ricardo C Rodríguez de la VegaLourival D Possani
Dec 7, 2007·Toxicon : Official Journal of the International Society on Toxinology·Najet Srairi-AbidMohamed El Ayeb
Jun 10, 2006·Toxicon : Official Journal of the International Society on Toxinology·Figen CaliskanLourival D Possani
Jan 2, 2007·Toxicon : Official Journal of the International Society on Toxinology·Paul A Whetstone, Bruce D Hammock
Feb 24, 2015·Toxicon : Official Journal of the International Society on Toxinology·A O CarmoE Kalapothakis
Jul 10, 2007·Toxicon : Official Journal of the International Society on Toxinology·John B OcholaIsaiah O Ndiege
Oct 24, 2007·Toxicon : Official Journal of the International Society on Toxinology·L H du PlessisJ L du Plessis
Jan 7, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Oren Froy, Michael Gurevitz
Aug 5, 2003·Journal of Biochemical and Molecular Toxicology·Cao ZhijianLi Wenxin
Jan 20, 2005·The Biochemical Journal·Najet Srairi-AbidMohamed El Ayeb
Dec 5, 2008·Chemical Research in Toxicology·Mary Gulumian
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Bora InceogluBruce D Hammock
Mar 9, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Zia NisaniWilliam K Hayes
Feb 22, 2011·Peptides·Najwa AbbasMarie-France Martin-Eauclaire

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