Isolation and characterization of a novel perivitellin from the eggs of Pomacea scalaris (Mollusca, Ampullariidae)

Molecular Reproduction and Development
Santiago ItuarteHoracio Heras

Abstract

Perivitellins are important components of the perivitelline fluid (PVF) that surrounds gastropod embryos. The glyco-lipo-carotenoprotein ovorubin (OR) from eggs of the snail Pomacea canaliculata has been the most studied to date. Here we report the characterization of scalarin (SC), a glyco-lipo-carotenoprotein from the PVF of P. scalaris. SC was purified by ultracentrifugation and exclusion chromatography. It is the major egg protein, representing 64% of the total soluble protein. The particle has a hydration density of 1.26 g/ml, an apparent molecular mass of 380 kDa and it is an elongated compact protein as estimated by small angle X-ray scattering (SAXS). It is composed of three subunits of ca. 35, 28, and 24 kDa noncovalently bonded. SC is highly glycosylated (carbohydrate content 20.1%, by wt.), with a low lipid content (0.7%), being esterified sterols, pigments and polar lipids the most abundant lipid classes. HPTLC and spectrophotometric analysis of the carotenoid fraction revealed the presence of free astaxanthin (ASX; 62.0%), and an unidentified carotenoid (38.0%). The carotenoid-apoprotein interaction was studied by spectrophotometry. Carotenoids do not seem to affect the structural characteristics of the oligomer. H...Continue Reading

References

Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P F ZagalskyJ B Findlay
Jan 1, 1985·Methods in Enzymology·P F Zagalsky
Aug 15, 1972·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D A Norden
Jan 1, 1995·Developmental and Comparative Immunology·R IijimaM Yamazaki
Dec 1, 1996·The Journal of Experimental Zoology·C F GarinR J Pollero
Jun 19, 1998·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·F MorishitaA S Saleuddin
Mar 7, 2003·Molecular and Cellular Biochemistry·Marcos S DreonRicardo J Pollero
Jun 5, 2003·FEBS Letters·Cosma D DellisantiVéronique Receveur-Bréchot
Dec 24, 1958·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D F CHEESMAN
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 17, 2004·Archives of Biochemistry and Biophysics·Marcos S DreonRicardo J Pollero
Apr 28, 2004·Molecular Reproduction and Development·Marcos S DreonRicardo J Pollero
Feb 27, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·H HerasR J Pollero
Feb 28, 2007·Archives of Biochemistry and Biophysics·Marcos S DreonHoracio Heras

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Citations

Nov 27, 2015·Developmental and Comparative Immunology·Olga Lucia BaronChristine Coustau
Jul 22, 2008·Toxicon : Official Journal of the International Society on Toxinology·Horacio HerasMarcos S Dreon
Nov 22, 2017·Scientific Reports·María Yanina PasquevichHoracio Heras
Oct 28, 2019·Rapid Communications in Mass Spectrometry : RCM·Jack C H IpJian-Wen Qiu
Mar 11, 2010·Biochimica Et Biophysica Acta·María Victoria FrassaHoracio Heras
May 18, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S ItuarteH Heras

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