Isolation and characterization of a novel ribonuclease from the pink oyster mushroom Pleurotus djamor

The Journal of General and Applied Microbiology
Xiangli WuTzi Bun Ng

Abstract

A 15-kDa RNase was purified from Pleurotus djamor using ion exchange chromatography and gel filtration. Its N-terminal amino acid sequence was different from previously reported RNase sequences of mushrooms belonging to the genus of Pleurotus and other genera. The RNase exhibited maximal RNase activity at pH 4.6 and 60 degrees C. Its activity toward polyhomoribonucleotides was poly(U) > poly(C) > poly(A) > poly(G). It inhibited proliferation of hepatoma cells and breast cancer cells. The ranking of inhibitory potencies of metal ions on RNase activity was Fe(3+)> Al(3+)> Ca(2+)> Hg(2+). The isolated RNase had a distinctive N-terminal sequence and optimum pH. It exhibited antiproliferative activity on tumor cells.

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Citations

Sep 17, 2010·Biochimica Et Biophysica Acta·Evandro Fei Fang, Tzi Bun Ng
May 2, 2018·Arab Journal of Urology·Damayanthi Durairajanayagam
Jun 3, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Tatiana Rodrigues AlexandreAndre Gustavo Tempone
Jun 20, 2020·Applied Microbiology and Biotechnology·Vala RezvaniLeila Farahmand

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