PMID: 2105290Feb 1, 1990Paper

Isolation and characterization of a novel extracellular metalloprotease from Bacillus subtilis

Journal of Bacteriology
G A RufoJ Pero

Abstract

We have isolated and characterized two minor extracellular proteases from culture supernatants of a strain of Bacillus subtilis containing deletion mutations of the genes for the extracellular proteases subtilisin (apr) and neutral protease (npr) and a minor extracellular protease (epr) as well as intracellular serine protease-I (isp-1). Characterization studies have revealed that one of these enzymes is the previously described protease bacillopeptidase F. The second enzyme, the subject of this report, is a novel metalloprotease, which we designate Mpr. Mpr is a unique metalloprotease that has been purified to apparent homogeneity by using both conventional and high-performance liquid chromatography procedures. Mpr has a molecular mass of approximately 28 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a basic isoelectric point of 8.7. The enzyme showed maximal activity against azocoll at pH 7.5 and 50 degrees C. Mpr was inhibited by dithiothreitol and a combination of beta-mercaptoethanol and EDTA. Activity was moderately inhibited by beta-mercaptoethanol and EDTA alone as well as by cysteine and citrate and only marginally by phosphoramidon 1,10-phenanthroline and N-[N-(L-3-trans-carboxyoxiran-2-...Continue Reading

References

Jan 1, 1976·Methods in Enzymology·G R Drapeau
Feb 1, 1990·Journal of Bacteriology·A SlomaJ Pero
Dec 1, 1988·Journal of Bacteriology·A SlomaJ Pero
Apr 1, 1973·The Biochemical Journal·D M Shotton, B S Hartley
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Feb 1, 1963·Journal of Bacteriology·R S HANSONH O HALVORSON

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Citations

Oct 15, 2014·Journal of Bacteriology·Rebecca A Calvo, Daniel B Kearns
Feb 11, 2015·Journal of Bacteriology·Giulia BarbieriBoris R Belitsky
May 29, 2018·Molecular Microbiology·Elliot ErskineCait E MacPhee
Dec 29, 1998·Protein Science : a Publication of the Protein Society·L YuT F Smith
Dec 14, 2002·Microbiology·Ritsuko KuwanaKazuhito Watabe
Sep 18, 2004·Journal of Bacteriology·Chi Hye ParkSi Myung Byun
Sep 1, 1991·Research in Microbiology·X S HeR H Doi
Feb 1, 1990·Journal of Bacteriology·A SlomaJ Pero
Oct 1, 1991·Current Opinion in Biotechnology·R H Doi
Dec 15, 2004·Protein Expression and Purification·Chang Won KhoByoung Chul Park
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva
Feb 15, 2007·Journal of Bioscience and Bioengineering·Takeko KodamaJunichi Sekiguchi
Sep 22, 2007·Journal of Bioscience and Bioengineering·Takeko KodamaJunichi Sekiguchi

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